In [1]:
import tifffile
from matplotlib import pyplot as plt
from skimage import (exposure, feature, filters, io, measure,
morphology, restoration, segmentation, transform,
util)
import numpy as np
from skimage.morphology import remove_small_objects, dilation, erosion, ball
from skimage.feature import peak_local_max
from scipy import ndimage as ndi
from skimage.measure import label
import cv2
#from aicssegmentation.core.seg_dot import dot_3d, dot_3d_wrapper
#from aicssegmentation.core.pre_processing_utils import intensity_normalization, image_smoothing_gaussian_slice_by_slice
import skimage.io
from skimage.measure import label, regionprops, regionprops_table
#import skimage.measure
import pandas as pd
from pathlib import Path
import glob
import seaborn as sns
import nd2
from skimage import img_as_uint
from skimage.util import invert
from vedo import Volume
import pytrax as pt
import pyvista as pv
import os
The folder "IMAGES" contains the imaging data. For each replicate there is the nd2 file, a coresponding "cell" mask and a "protein" mask.¶
In [2]:
os.chdir('G:\\D\\AMR_PHD_PROJECT\\OUT\\')
rootdir = Path('G:\\D\\AMR_PHD_PROJECT\\IMAGES')
file_list = [f for f in rootdir.resolve().glob('**/*') if f.is_file()]
file_list=pd.DataFrame(data=file_list)
file_list.columns = ['Data_Path']
file_list = file_list.applymap(str)
file_list['TEXT_1'] = file_list['Data_Path'].str.lstrip(r"G:\\D\AMR_PHD_PROJECT\IMAGES\Mouse\*")
file_list=file_list.assign(IMAGE_ID=file_list['TEXT_1'].str.extract('(.*)M'))
file_list['IMAGE_ID'] = file_list['IMAGE_ID'].str.replace('\\', '')
file_list['IMAGE_ID'] = "MS" + file_list['IMAGE_ID']
file_list=file_list.dropna()
UNIQUE_ID =np.unique(file_list['IMAGE_ID'].astype(str))
out='G:\\D\\AMR_PHD_PROJECT\\OUT\\'
ref_df = pd.read_csv('G:\\D\\AMR_PHD_PROJECT\\sod1_mouse_ref.csv')
#141-ROI3 eg of image name
ref_df['ID'] = ref_df['Folder name'].astype(str)
ref_df['IMAGE_ID'] = ref_df['Folder name']
Create empty folders to hold information as the loop runs¶
In [3]:
Cell_Volume = []
Void_Volume = []
Protein_in_Cell_Volume = []
Protein_in_Void_Volume = []
Run the image processing steps¶
In [4]:
for ID in UNIQUE_ID:
ID_BOOL=file_list.IMAGE_ID == ID
SUB_DF = file_list[ID_BOOL]
ID_TXT= ID
ID_BOOL_ref=ref_df.IMAGE_ID == ID
ref_sub = ref_df[ID_BOOL_ref]
Genotype_id=ref_sub['Genotype']
Genotype_id_string=str(np.array(Genotype_id))
#Read in Cells
cell_df = SUB_DF[SUB_DF['TEXT_1'].str.contains("cells")]
cell_path=(cell_df.Data_Path)
cell_path=np.array(cell_path)
cell_img=tifffile.imread(cell_path[0])
print("Images from folder")
print(cell_df.IMAGE_ID)
print("shape of image Z,X, Y:" )
print(cell_img.shape)
print("Number of unique cells:")
print(np.unique(cell_img))
nd2_df=SUB_DF[SUB_DF['TEXT_1'].str.contains("nd2")]
nd2_path=(nd2_df.Data_Path)
nd2_path=np.array(nd2_path)
nd2_img=nd2.ND2File(nd2_path[0])
#extract voxel size from nd2 raw file
voxel_size=nd2_img.voxel_size()
X_VOX_SIZE =voxel_size[0]
Y_VOX_SIZE =voxel_size[1]
Z_VOX_SIZE =voxel_size[2]
print(nd2_img.voxel_size())
#Read in protein
protein_df = SUB_DF[SUB_DF['TEXT_1'].str.contains("protein")]
protein_path=(protein_df.Data_Path)
protein_path=np.array(protein_path)
protein=tifffile.imread(protein_path[0])
#Cells are segmented indivdually, here we make them binary
cells_binary=cell_img.copy()
cells_binary[cells_binary !=0 ] = 1
#Void is any "0" pixel in the cell 3D stack
void_binary=cell_img.copy()
void_binary = (void_binary == 0).astype(np.uint8)
# using "*" essentially locks the object to its source, so only the protein in cells or void below
protein_in_cell = cells_binary * protein
protein_in_void = void_binary * protein
#Create STL's using Vedo and Plot using Pyvista
CELL_VOL = Volume(cells_binary, spacing=(Z_VOX_SIZE,Y_VOX_SIZE,X_VOX_SIZE))
CELL_ISO = CELL_VOL.isosurface(1)
CELL_ISO.write(ID+"CELLS.stl")
CELL_VOL_STL=pv.read(ID+"CELLS.stl")
PROTEIN_VOL = Volume(protein, spacing=(Z_VOX_SIZE,Y_VOX_SIZE,X_VOX_SIZE))
PROTEIN_ISO = PROTEIN_VOL.isosurface(1)
PROTEIN_ISO.write(ID+"ALL_PROTEIN.stl")
PROTEIN_VOL_STL=pv.read(ID+"ALL_PROTEIN.stl")
CELL_PROTEIN_VOL = Volume(protein_in_cell, spacing=(Z_VOX_SIZE,Y_VOX_SIZE,X_VOX_SIZE))
CELL_PROTEIN_ISO = CELL_PROTEIN_VOL.isosurface(1)
CELL_PROTEIN_ISO.write(ID+"CELL_PROTEIN.stl")
CELL_PROTEIN_STL=pv.read(ID+"CELL_PROTEIN.stl")
VOID_PROTEIN_VOL = Volume(protein_in_void, spacing=(Z_VOX_SIZE,Y_VOX_SIZE,X_VOX_SIZE))
VOID_PROTEIN_ISO = VOID_PROTEIN_VOL.isosurface(1)
VOID_PROTEIN_ISO.write(ID+"VOID_PROTEIN.stl")
VOID_PROTEIN_STL=pv.read(ID+"VOID_PROTEIN.stl")
#Need an if else statement for when there is no protein
if PROTEIN_VOL_STL.n_points != 0:
pv.set_plot_theme("document")
p = pv.Plotter()
p.add_mesh(CELL_VOL_STL, color="grey", opacity=0.4).rotate_y(90);
p.add_title(Genotype_id_string)
p.add_mesh(PROTEIN_VOL_STL, color="red", opacity=1).rotate_y(90);
p.window_size = 500, 500
p.show(jupyter_backend='static');
p.screenshot(ID+Genotype_id_string+'3D_VIS.png') ;
else:
pv.set_plot_theme("document")
p = pv.Plotter()
p.add_mesh(CELL_VOL_STL, color="grey", opacity=0.4).rotate_y(90);
p.add_title(Genotype_id_string)
p.window_size = 500, 500
p.show(jupyter_backend='static')
p.screenshot(ID+Genotype_id_string+'3D_VIS.png') ;
#use regions props to extract volume (um^3)
Protein_Labels = label((protein_in_cell))
PROTEIN_INFO = skimage.measure.regionprops_table(Protein_Labels, intensity_image=cell_img, properties = ('label','area', 'intensity_max'))
PROTEIN_INFO=pd.DataFrame(PROTEIN_INFO)
PROTEIN_INFO.columns = ['Protein_Label', 'Protein_Area_Pixels', "Cell_Label"]
PROTEIN_INFO['Proten_Volume_Voxel_Resolved'] = PROTEIN_INFO['Protein_Area_Pixels'] * X_VOX_SIZE *Y_VOX_SIZE*Z_VOX_SIZE
PROTEIN_INFO["ID"] = ID
Protein_in_Cell_Volume.append(PROTEIN_INFO)
print("Protein Volume Inside Cells'")
print(PROTEIN_INFO['Proten_Volume_Voxel_Resolved'])
Protein_Labels_void = label((protein_in_void))
PROTEIN_INFO_void = skimage.measure.regionprops_table(Protein_Labels_void, properties = ('label','area'))
PROTEIN_INFO_void=pd.DataFrame(PROTEIN_INFO_void)
PROTEIN_INFO_void.columns = ['Protein_Label', 'Protein_Area_Pixels']
PROTEIN_INFO_void['Proten_Volume_Voxel_Resolved'] = PROTEIN_INFO_void['Protein_Area_Pixels'] * X_VOX_SIZE *Y_VOX_SIZE*Z_VOX_SIZE
PROTEIN_INFO_void["ID"] = ID
Protein_in_Void_Volume.append(PROTEIN_INFO_void)
print("Protein Volume Inside Void'")
print(PROTEIN_INFO_void['Proten_Volume_Voxel_Resolved'])
CELL_INFO = skimage.measure.regionprops_table(cell_img, properties = ('label','area'))
CELL_INFO=pd.DataFrame(CELL_INFO)
CELL_INFO.columns = ['Cell_Label', 'Cell_Area_Pixels', ]
CELL_INFO['Cell_Volume_Voxel_Resolved'] = CELL_INFO['Cell_Area_Pixels'] * X_VOX_SIZE *Y_VOX_SIZE*Z_VOX_SIZE
CELL_INFO["ID"] = ID
Cell_Volume.append(CELL_INFO)
print("Cell Volume")
print(CELL_INFO['Cell_Volume_Voxel_Resolved'])
VOID_INFO = skimage.measure.regionprops_table(void_binary, properties = ('label','area'))
VOID_INFO=pd.DataFrame(VOID_INFO)
VOID_INFO.columns = ['Void_Label', 'Void_Area_Pixels', ]
VOID_INFO['Void_Volume_Voxel_Resolved'] = VOID_INFO['Void_Area_Pixels'] * X_VOX_SIZE *Y_VOX_SIZE*Z_VOX_SIZE
VOID_INFO["ID"] = ID
Void_Volume.append(VOID_INFO)
print("Void Volume")
print(VOID_INFO['Void_Volume_Voxel_Resolved'])
print("Move On to Next Image")
print("No more images left")
Images from folder 1 MS100-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (16, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 12 13 14 15 16 17 18 19] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.062714 1 0.021950 2 0.012543 3 0.116020 4 0.144242 5 0.018814 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.078392 1 0.003136 2 0.081528 3 0.003136 4 0.012543 5 0.018814 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 356.458791 1 806.499069 2 411.863270 3 587.697000 4 882.950289 5 1218.346661 6 225.791518 7 841.995062 8 151.572908 9 402.192807 10 646.873713 11 928.659219 12 447.732411 13 451.429387 14 412.286588 15 386.984718 16 130.877364 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 43317.911612 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 4 MS100-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (15, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 11 12 13 14 15 16 17 18 19 20] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 2.489737 1 0.025086 2 0.006271 3 0.047035 4 0.015678 5 0.094071 6 0.037628 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.043900 1 0.003136 2 0.003136 3 0.006271 4 0.006271 5 0.003136 6 0.003136 7 0.003136 8 0.003136 9 0.003136 10 0.003136 11 0.006271 12 0.003136 13 0.003136 14 0.003136 15 0.006271 16 0.003136 17 0.053307 18 0.003136 19 0.018814 20 0.015678 21 0.003136 22 0.003136 23 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 447.475284 1 747.140486 2 691.980591 3 375.376400 4 484.899726 5 526.529126 6 310.085095 7 654.534199 8 416.021193 9 67.984861 10 182.688345 11 110.846587 12 123.113400 13 172.616513 14 242.363631 15 949.633839 16 440.821353 17 182.813772 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 42193.190337 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 7 MS101-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (18, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.009407 1 0.125428 2 0.279076 3 0.109749 4 0.122292 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.025086 1 0.003136 2 0.012543 3 0.006271 4 0.159920 5 0.003136 6 0.003136 7 0.015678 8 0.047035 9 0.159920 10 0.147377 11 0.009407 12 0.025086 13 0.053307 14 0.003136 15 0.003136 16 0.006271 17 0.169327 18 0.003136 19 0.021950 20 0.464082 21 0.021950 22 1.567844 23 0.003136 24 0.043900 25 0.009407 26 0.116020 27 0.025086 28 0.003136 29 0.003136 30 0.012543 31 0.015678 32 0.018814 33 0.015678 34 0.018814 35 0.012543 36 0.025086 37 0.012543 38 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 489.324182 1 889.782954 2 859.394997 3 1110.435078 4 1206.145697 5 884.420927 6 704.930984 7 118.980562 8 182.562917 9 614.243739 10 749.156734 11 1039.505806 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 50335.253109 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 11 MS101-7_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (18, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.037628 1 0.059578 2 0.028221 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.072121 1 0.003136 2 0.003136 3 0.106613 4 0.003136 5 0.059578 6 0.059578 7 0.006271 8 0.003136 9 0.003136 10 0.147377 11 0.006271 12 0.153649 13 0.028221 14 0.025086 15 0.219498 16 0.075257 17 0.247719 18 0.181870 19 0.100342 20 0.059578 21 0.012543 22 0.012543 23 0.075257 24 0.056442 25 0.075257 26 0.025086 27 0.009407 28 0.006271 29 0.006271 30 0.003136 31 0.006271 32 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 955.748432 1 1283.205241 2 1053.619539 3 1123.796247 4 1018.575085 5 662.147651 6 1728.360115 7 176.451460 8 858.767859 9 435.547125 10 219.896424 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 49668.022507 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 15 MS102-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (15, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.357468
1 0.263398
2 0.056442
3 0.103478
4 0.021950
...
63 0.006271
64 0.003136
65 0.003136
66 0.003136
67 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 68, dtype: float64
Cell Volume
0 946.585950
1 736.946363
2 725.419572
3 247.672352
4 602.927039
5 398.649479
6 293.973928
7 299.382990
8 319.197405
9 55.661606
10 270.130153
11 369.089344
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 44054.478557
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
18 MS102-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(18, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.006271
1 0.003136
2 0.009407
3 0.009407
4 0.006271
...
100 0.395097
101 0.034493
102 0.003136
103 0.003136
104 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 105, dtype: float64
Cell Volume
0 837.282122
1 560.033957
2 856.735933
3 274.325704
4 241.545217
5 1056.404031
6 878.227942
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 54479.582781
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
21 MS106(273)-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(37, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 16 17 18 19 20 21 22 23 24 25
26]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.015678
1 0.021950
2 0.009407
3 0.021950
4 0.072121
...
129 0.062714
130 0.009407
131 0.018814
132 0.037628
133 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 134, dtype: float64
Protein Volume Inside Void'
0 0.065849
1 0.395097
2 0.075257
3 0.009407
4 0.034493
...
66 0.028221
67 0.109749
68 0.003136
69 0.003136
70 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 71, dtype: float64
Cell Volume
0 968.711368
1 1301.204092
2 2422.811629
3 1637.262094
4 1025.188252
5 892.915507
6 935.388407
7 1659.403191
8 1754.129203
9 1413.314361
10 1096.650592
11 779.764189
12 1264.331532
13 1267.912488
14 1843.041649
15 1880.945851
16 1823.095535
17 1102.050248
18 1107.772879
19 176.592566
20 426.541428
21 324.838509
22 1631.846761
23 520.198171
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 92400.372523
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
24 MS106(273)-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(29, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.062714
1 0.075257
2 0.003136
3 0.072121
4 0.040764
5 0.003136
6 0.072121
7 0.009407
8 0.056442
9 0.050171
10 0.307297
11 0.021950
12 0.163056
13 0.213227
14 0.244584
15 0.003136
16 0.172463
17 0.153649
18 0.072121
19 0.131699
20 0.025086
21 0.028221
22 0.159920
23 0.037628
24 0.119156
25 0.137970
26 0.056442
27 0.025086
28 0.175599
29 0.125428
30 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.442132
1 0.137970
2 0.012543
3 0.040764
4 0.009407
...
71 0.003136
72 0.018814
73 0.003136
74 0.003136
75 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 76, dtype: float64
Cell Volume
0 103.283306
1 88.975160
2 1725.973856
3 1789.205014
4 2609.008809
5 2339.543422
6 248.898406
7 208.366497
8 1506.983647
9 1869.632287
10 561.824435
11 2181.868464
12 632.110891
13 1530.689451
14 499.653140
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 77456.205043
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
27 MS108-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(26, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.953249
1 0.003136
2 0.047035
3 0.313569
4 0.009407
5 0.043900
6 0.078392
7 0.025086
8 0.003136
9 0.065849
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.573831
1 3.364594
2 0.498574
3 0.175599
4 0.790193
...
462 0.009407
463 0.003136
464 0.003136
465 0.006271
466 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 467, dtype: float64
Cell Volume
0 2720.121929
1 1587.382698
2 495.470132
3 2085.957161
4 421.091602
5 1007.336778
6 93.882512
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 77076.956068
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
30 MS108-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(22, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.003136
2 0.006271
3 0.037628
4 0.068985
5 0.006271
6 0.009407
7 0.667902
8 0.332383
9 0.075257
10 0.018814
11 0.153649
12 0.178734
13 0.633409
14 0.009407
15 0.379418
16 0.006271
17 0.003136
18 0.003136
19 0.047035
20 0.272805
21 0.003136
22 0.025086
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.191277
1 0.062714
2 0.006271
3 0.188141
4 0.009407
...
690 0.021950
691 0.003136
692 0.006271
693 0.009407
694 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 695, dtype: float64
Cell Volume
0 961.038338
1 981.238443
2 881.326002
3 1267.442135
4 357.832223
5 795.831457
6 167.646447
7 687.998267
8 1395.804677
9 1846.801339
10 241.404111
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 62751.804845
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
33 MS110-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(34, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.630273
1 0.065849
2 0.003136
3 0.084664
4 0.031357
5 0.003136
6 0.012543
7 0.363740
8 0.047035
9 0.034493
10 0.028221
11 0.031357
12 0.081528
13 0.191277
14 0.003136
15 0.103478
16 0.043900
17 0.003136
18 0.003136
19 0.006271
20 0.012543
21 0.025086
22 0.012543
23 0.003136
24 0.172463
25 0.025086
26 0.125428
27 0.025086
28 0.285348
29 0.009407
30 0.413911
31 0.040764
32 0.003136
33 0.018814
34 0.517389
35 0.018814
36 0.003136
37 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.043900
1 0.263398
2 0.034493
3 0.112885
4 0.018814
...
1548 0.003136
1549 0.003136
1550 0.003136
1551 0.003136
1552 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 1553, dtype: float64
Cell Volume
0 2103.495067
1 2545.815279
2 2085.731392
3 2764.999902
4 256.809748
5 289.910075
6 2960.463041
7 100.787298
8 91.383368
9 2337.897185
10 153.852554
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 96101.115164
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
36 MS110-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(42, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 13 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.899943
1 0.078392
2 0.053307
3 0.216363
4 0.009407
5 0.003136
6 0.006271
7 0.003136
8 0.003136
9 0.003136
10 0.003136
11 0.012543
12 0.009407
13 0.056442
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 29.923875
1 17.105180
2 450.084177
3 0.222634
4 0.134835
...
688 0.003136
689 0.003136
690 0.003136
691 0.144242
692 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 693, dtype: float64
Cell Volume
0 115.173837
1 145.075762
2 1605.149509
3 248.246183
4 300.618451
5 424.754086
6 60.766506
7 44.620847
8 55.260238
9 88.602013
10 1327.979736
11 1523.891279
12 1528.227936
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 130627.954887
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
39 MS111-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(32, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.068985
2 0.009407
3 0.006271
4 0.078392
5 0.006271
6 0.279076
7 0.021950
8 0.006271
9 0.003136
10 0.103478
11 0.003136
12 0.068985
13 0.003136
14 0.028221
15 0.006271
16 0.006271
17 0.037628
18 0.097206
19 0.053307
20 0.021950
21 0.072121
22 0.037628
23 0.065849
24 0.012543
25 0.003136
26 0.018814
27 0.003136
28 0.006271
29 0.025086
30 0.003136
31 0.018814
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.373147
1 1.514538
2 0.163056
3 0.003136
4 0.040764
...
1564 0.003136
1565 0.081528
1566 0.012543
1567 0.003136
1568 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 1569, dtype: float64
Cell Volume
0 411.389782
1 296.601635
2 110.476575
3 1475.654983
4 777.268181
5 856.190323
6 1427.174104
7 1143.011746
8 497.085011
9 1177.915094
10 210.595972
11 1607.284913
12 140.569777
13 110.175549
14 428.203343
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 94546.647787
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
42 MS111-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(27, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.003136
2 0.003136
3 0.003136
4 0.003136
...
69 0.219498
70 0.003136
71 0.009407
72 0.003136
73 0.031357
Name: Proten_Volume_Voxel_Resolved, Length: 74, dtype: float64
Protein Volume Inside Void'
0 1.041049
1 0.021950
2 0.003136
3 0.003136
4 0.003136
...
1288 0.003136
1289 0.003136
1290 0.009407
1291 0.003136
1292 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 1293, dtype: float64
Cell Volume
0 2057.961735
1 1697.144337
2 1336.452366
3 378.725315
4 1940.254262
5 2292.134948
6 1304.527922
7 1207.437601
8 469.346711
9 883.652683
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 75208.56865
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
46 MS112-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(27, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.050171 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.003136 2 0.028221 3 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 254.636716 1 216.669800 2 919.195711 3 479.211587 4 665.888527 5 54.852598 6 829.728249 7 811.550663 8 720.007374 9 430.429682 10 635.801597 11 906.878727 12 282.005005 13 115.042138 14 119.548122 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 81334.760033 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 49 MS112-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1569.992170 1 1102.997225 2 1497.833708 3 1233.573564 4 1289.699251 5 930.653517 6 1493.277552 7 698.186118 8 216.785821 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 55727.154058 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 52 MS114-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.075257
1 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.068985
2 0.178734
3 0.100342
4 0.031357
...
399 0.003136
400 0.006271
401 0.009407
402 0.009407
403 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 404, dtype: float64
Cell Volume
0 877.478512
1 477.038555
2 795.800100
3 1914.958663
4 50.089487
5 1674.993834
6 547.757736
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 49558.01315
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
55 MS114-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.081528
1 0.006271
2 0.006271
3 0.307297
4 0.003136
5 0.003136
6 0.021950
7 0.238312
8 0.015678
9 0.003136
10 0.018814
11 0.006271
12 0.006271
13 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.015678
1 0.037628
2 0.003136
3 0.003136
4 0.003136
...
570 0.003136
571 0.009407
572 0.006271
573 0.003136
574 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 575, dtype: float64
Cell Volume
0 2263.499841
1 1496.306627
2 208.905836
3 1854.047915
4 1417.168122
5 1337.568671
6 853.766436
7 1039.725304
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 55289.164231
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
58 MS115-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(16, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
25 26]
VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.062714
1 5.217786
2 0.075257
3 0.012543
4 0.012543
5 0.050171
6 0.137970
7 0.012543
8 0.087799
9 0.037628
10 0.062714
11 0.050171
12 0.062714
13 0.100342
14 0.025086
15 0.012543
16 0.150513
17 0.037628
18 0.025086
19 0.012543
20 0.050171
21 0.012543
22 0.012543
23 2.194982
24 0.025086
25 0.137970
26 0.376283
27 0.050171
28 0.025086
29 0.025086
30 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.025086
2 0.050171
3 0.551881
4 0.012543
...
942 0.012543
943 0.012543
944 0.012543
945 0.012543
946 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 947, dtype: float64
Cell Volume
0 831.572033
1 786.129636
2 763.866248
3 1057.078204
4 619.737465
5 685.436409
6 531.285965
7 140.616813
8 507.667960
9 96.541576
10 883.637004
11 1199.438459
12 1062.634644
13 1018.584492
14 938.887835
15 1201.457843
16 178.809498
17 784.674677
18 717.320089
19 723.478581
20 475.094428
21 869.237923
22 956.987029
23 804.692912
24 197.912112
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 192399.709715
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
61 MS115-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
25 26 27]
VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.037628
2 0.213227
3 0.112885
4 0.112885
5 0.025086
6 0.012543
7 0.075257
8 0.137970
9 0.012543
10 0.012543
11 0.025086
12 0.037628
13 0.288483
14 0.012543
15 0.163056
16 0.012543
17 0.175599
18 0.012543
19 0.012543
20 0.025086
21 0.150513
22 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.915621
2 0.025086
3 0.338654
4 0.012543
...
1036 0.012543
1037 0.012543
1038 0.012543
1039 0.012543
1040 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 1041, dtype: float64
Cell Volume
0 2303.276049
1 523.246060
2 1653.686831
3 1266.918475
4 1740.269460
5 380.095611
6 137.995377
7 148.995372
8 152.143603
9 648.924453
10 357.970193
11 821.751057
12 951.756700
13 873.301775
14 609.778518
15 957.476196
16 205.914389
17 81.527900
18 566.230077
19 840.602816
20 890.748746
21 577.631440
22 1124.508048
23 1184.713266
24 354.746705
25 973.355322
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 242713.047498
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
64 MS126-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(26, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 16 17 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.012543 1 0.006271 2 0.040764 3 0.078392 4 0.043900 5 0.053307 6 0.081528 7 0.006271 8 0.040764 9 0.012543 10 0.003136 11 0.122292 12 0.053307 13 0.018814 14 0.021950 15 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.009407 1 0.025086 2 0.106613 3 0.009407 4 0.006271 5 0.006271 6 0.003136 7 0.062714 8 0.417047 9 0.018814 10 0.047035 11 0.025086 12 0.147377 13 0.006271 14 0.018814 15 0.009407 16 0.021950 17 0.003136 18 0.006271 19 0.006271 20 0.006271 21 0.037628 22 0.053307 23 0.006271 24 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1848.171635 1 2173.160657 2 1725.114678 3 2214.028085 4 1039.261222 5 432.358130 6 311.449119 7 1174.519143 8 2022.192937 9 1105.207886 10 1388.718021 11 766.895324 12 210.128754 13 187.156701 14 628.219502 15 481.782851 16 259.860773 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 67519.973462 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 67 MS126-8_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (23, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.257126
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.062714
2 0.012543
3 1.273090
4 0.834093
...
58 0.053307
59 0.050171
60 0.034493
61 0.018814
62 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 63, dtype: float64
Cell Volume
0 1308.535332
1 118.601144
2 47.352031
3 188.188342
4 1764.386040
5 1877.377437
6 1838.548207
7 1452.497924
8 129.983693
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 66898.705781
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
70 MS128-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.376283
1 0.003136
2 0.072121
3 0.009407
4 0.050171
...
70 0.003136
71 0.059578
72 0.003136
73 0.003136
74 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 75, dtype: float64
Protein Volume Inside Void'
0 2.129132
1 0.783922
2 0.881128
3 2.295324
4 0.865450
...
2044 0.006271
2045 0.056442
2046 0.012543
2047 0.028221
2048 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 2049, dtype: float64
Cell Volume
0 1304.082654
1 41.312695
2 1419.422682
3 250.121325
4 1192.427060
5 177.091141
6 886.339968
7 71.521918
8 436.020614
9 259.362199
10 686.254824
11 50.550433
12 246.957415
13 646.506837
14 22.981461
15 1104.329893
16 241.457418
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 89603.488941
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
73 MS128-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
25 26]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.043900
1 0.009407
2 0.006271
3 0.119156
4 0.015678
...
171 0.003136
172 0.031357
173 0.006271
174 0.009407
175 0.015678
Name: Proten_Volume_Voxel_Resolved, Length: 176, dtype: float64
Protein Volume Inside Void'
0 2.016248
1 0.473489
2 0.943842
3 0.517389
4 0.238312
...
2251 0.062714
2252 0.003136
2253 0.003136
2254 0.003136
2255 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 2256, dtype: float64
Cell Volume
0 309.994160
1 1699.282876
2 461.335027
3 233.169793
4 1126.044536
5 342.743290
6 90.502240
7 516.218982
8 1500.458279
9 625.246869
10 782.197483
11 780.736252
12 626.269104
13 200.709146
14 142.871373
15 1445.574324
16 364.432847
17 169.496503
18 880.172069
19 818.819189
20 153.818061
21 841.358517
22 367.690827
23 318.742731
24 820.829165
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 60005.46229
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
76 MS129-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.235177 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.006271 2 0.003136 3 1.705815 4 0.012543 5 0.357468 6 0.006271 7 0.025086 8 0.003136 9 0.043900 10 0.009407 11 0.003136 12 0.003136 13 0.003136 14 0.686716 15 0.018814 16 0.003136 17 0.003136 18 0.642816 19 0.250855 20 0.003136 21 0.012543 22 2.370580 23 0.018814 24 0.097206 25 0.037628 26 0.003136 27 0.166191 28 0.015678 29 0.003136 30 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 998.955083 1 1196.199293 2 1561.623018 3 2287.961347 4 259.196007 5 1345.081781 6 894.630728 7 1457.825459 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 55758.68027 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 79 MS129-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.119156
1 0.003136
2 0.094071
3 0.018814
4 0.103478
5 0.003136
6 0.003136
7 0.003136
8 0.009407
9 0.003136
10 0.028221
11 0.021950
12 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.006271
2 0.250855
3 0.003136
4 0.003136
...
147 0.003136
148 0.003136
149 0.003136
150 0.009407
151 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 152, dtype: float64
Cell Volume
0 1099.397455
1 1313.094623
2 1164.481805
3 3303.488543
4 1532.552050
5 1297.563558
6 643.929301
7 428.404027
8 297.206822
9 354.530343
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 54325.504458
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
81 MS131-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.050171
1 0.015678
2 0.056442
3 0.037628
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.028221
3 0.025086
4 0.404504
...
98 0.047035
99 0.081528
100 0.009407
101 0.009407
102 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 103, dtype: float64
Cell Volume
0 1558.120454
1 1898.370871
2 1257.539631
3 1573.030652
4 1639.883530
5 342.994145
6 447.450199
7 2079.460015
8 793.859108
9 1669.199081
10 993.019225
11 489.963863
12 494.974693
13 942.929737
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 49579.357781
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
85 MS131-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(17, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.047035 1 1.755986 2 0.028221 3 0.012543 4 0.216363 5 0.018814 6 0.282212 7 0.003136 8 0.206955 9 0.025086 10 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 906.170062 1 1240.026811 2 1363.450644 3 583.115759 4 1523.828565 5 925.645823 6 829.220267 7 1285.785912 8 458.939361 9 301.471359 10 659.381974 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 45819.093502 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 89 MS135-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.043900 1 0.003136 2 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.059578 1 0.141106 2 0.003136 3 0.040764 4 0.018814 5 0.025086 6 0.304162 7 0.181870 8 0.504846 9 0.025086 10 0.021950 11 0.028221 12 0.034493 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 791.250216 1 91.681259 2 1618.824246 3 1312.824954 4 1101.830749 5 1157.834145 6 618.969221 7 290.333393 8 1043.453638 9 962.226764 10 221.473675 11 145.862819 12 240.033815 13 614.293910 14 738.420137 15 674.680998 16 1661.823942 17 151.685793 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 68762.687558 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 93 MS135-7_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (18, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.028221 1 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.021950 2 0.003136 3 0.003136 4 0.003136 5 0.090935 6 0.003136 7 0.003136 8 0.003136 9 0.003136 10 0.006271 11 0.050171 12 0.003136 13 0.003136 14 0.003136 15 0.003136 16 0.006271 17 0.006271 18 0.003136 19 0.003136 20 0.015678 21 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 565.794216 1 1230.644831 2 866.967685 3 431.524037 4 1310.560987 5 1210.102936 6 921.983338 7 856.522706 8 604.585818 9 1073.882358 10 706.495693 11 1144.212714 12 641.442700 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 47619.417667 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 97 MS141-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (34, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
2 0.078392
3 0.065849
4 0.003136
5 0.043900
6 0.003136
7 0.003136
8 0.006271
9 0.003136
10 0.009407
11 0.003136
12 0.003136
13 0.003136
14 0.031357
15 0.003136
16 0.043900
17 0.006271
18 0.263398
19 0.015678
20 0.012543
21 0.006271
22 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.846636
1 0.316705
2 3.772233
3 1.291904
4 1.850056
...
711 0.003136
712 0.031357
713 0.003136
714 0.009407
715 0.075257
Name: Proten_Volume_Voxel_Resolved, Length: 716, dtype: float64
Cell Volume
0 1311.852890
1 482.761186
2 1994.627100
3 1387.407303
4 1239.888840
5 1319.381678
6 755.901600
7 495.479539
8 103.716031
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 102701.243907
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
100 MS141-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 12 13 14 17 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.075257
1 0.034493
2 0.025086
3 0.037628
4 0.031357
5 0.003136
6 0.003136
7 0.012543
8 0.015678
9 0.006271
10 0.003136
11 0.012543
12 0.003136
13 0.003136
14 0.025086
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.721208
1 7.942699
2 0.373147
3 2.031926
4 0.040764
...
711 0.003136
712 0.006271
713 0.003136
714 0.009407
715 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 716, dtype: float64
Cell Volume
0 2129.010164
1 1102.852984
2 1637.763805
3 52.052428
4 949.414341
5 617.222643
6 418.833906
7 157.524445
8 573.043928
9 98.670708
10 117.102285
11 49.139374
12 277.928610
13 519.909687
14 236.255310
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 66687.451315
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
103 MS143-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(42, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.043900 1 0.021950 2 0.348061 3 0.006271 4 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.097206 1 0.053307 2 0.043900 3 0.078392 4 0.128563 5 0.012543 6 0.003136 7 0.050171 8 0.018814 9 0.175599 10 0.009407 11 0.348061 12 0.003136 13 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 803.824326 1 1626.494140 2 412.217603 3 423.565660 4 1814.036531 5 125.600001 6 1512.342538 7 849.467407 8 318.162628 9 1643.081932 10 1222.633147 11 1922.741442 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 125422.153912 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 106 MS143-8_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (31, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.062714 1 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2266.503831 1 884.405248 2 2348.263771 3 262.842813 4 1846.236915 5 765.299258 6 97.867972 7 1703.776318 8 1239.882569 9 404.572795 10 966.133832 11 1278.432723 12 974.126702 13 1086.020608 14 860.363925 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 84943.507847 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 109 MS144-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (26, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.043900 1 0.018814 2 0.062714 3 0.021950 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.009407 1 0.003136 2 0.009407 3 0.112885 4 0.021950 5 0.012543 6 0.081528 7 0.053307 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 768.964878 1 2479.774545 2 1187.472672 3 617.247728 4 145.514758 5 721.211479 6 729.455203 7 270.406093 8 946.567136 9 37.546733 10 310.257558 11 134.279586 12 357.111015 13 1436.844567 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 75345.544928 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 112 MS144-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (34, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.003136 2 0.003136 3 0.009407 4 0.006271 5 0.003136 6 0.015678 7 0.003136 8 0.003136 9 0.003136 10 0.003136 11 0.009407 12 0.006271 13 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1405.569211 1 1408.830327 2 439.087318 3 458.585028 4 398.144634 5 1204.744044 6 416.337898 7 431.464459 8 342.840496 9 541.009735 10 136.995093 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 104608.651832 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 115 MS146-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (19, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.043900 1 0.003136 2 0.018814 3 0.003136 4 0.047035 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.065849 1 0.006271 2 0.031357 3 0.003136 4 0.009407 5 0.025086 6 0.003136 7 0.003136 8 0.003136 9 0.003136 10 0.003136 11 0.006271 12 0.003136 13 0.006271 14 0.003136 15 0.003136 16 0.003136 17 0.009407 18 0.003136 19 0.003136 20 0.003136 21 0.018814 22 0.003136 23 0.003136 24 0.003136 25 0.031357 26 0.003136 27 0.003136 28 0.003136 29 0.003136 30 0.003136 31 0.009407 32 0.003136 33 0.003136 34 0.003136 35 0.003136 36 0.003136 37 0.003136 38 0.003136 39 0.006271 40 0.006271 41 0.012543 42 0.015678 43 0.034493 44 0.003136 45 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1629.400924 1 1134.024863 2 1052.478149 3 650.602046 4 669.767374 5 1296.422167 6 1397.579477 7 202.599966 8 182.519017 9 308.140968 10 214.173792 11 733.741690 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 53000.694903 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 118 MS146-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.006271 1 0.003136 2 0.015678 3 0.003136 4 0.090935 5 0.003136 6 0.003136 7 0.003136 8 0.003136 9 0.003136 10 0.003136 11 0.003136 12 0.003136 13 0.003136 14 0.018814 15 0.012543 16 0.009407 17 0.006271 18 0.009407 19 0.003136 20 0.003136 21 0.003136 22 0.003136 23 0.003136 24 0.003136 25 0.003136 26 0.003136 27 0.003136 28 0.003136 29 0.003136 30 0.003136 31 0.003136 32 0.003136 33 0.003136 34 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2290.087344 1 2158.614198 2 1981.940104 3 2398.958447 4 886.659808 5 371.036607 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 72112.894723 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 121 MS147-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8 9] VoxelSize(x=0.10182914864199052, y=0.10182914864199052, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Cell Volume 0 1326.496479 1 1311.857277 2 481.146334 3 2975.503350 4 870.581459 5 857.152340 6 2975.960631 7 3161.171733 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 67586.644768 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 124 MS147-7_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (27, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8 9] VoxelSize(x=0.10182914864199052, y=0.10182914864199052, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.326629 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1171.179710 1 379.259853 2 2197.945838 3 1117.914292 4 1949.480691 5 1509.050998 6 845.057734 7 461.806785 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 78438.53962 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 127 MS149-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (34, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.934435 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2684.629071 1 2204.219651 2 1164.776559 3 349.215351 4 146.427243 5 237.271273 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 105005.720924 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 130 MS149-9_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (32, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.028221 1 0.003136 2 0.059578 3 0.087799 4 0.018814 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 303.998724 1 564.815882 2 652.508545 3 329.018382 4 1612.242436 5 1576.467367 6 630.991451 7 853.967120 8 1012.654906 9 1118.584733 10 353.975326 11 499.960437 12 1032.055410 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 94675.004058 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 133 MS150-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (30, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 15 16 17 18] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.388825
1 0.003136
2 0.043900
3 0.188141
4 0.075257
...
174 0.003136
175 0.015678
176 0.009407
177 0.094071
178 0.081528
Name: Proten_Volume_Voxel_Resolved, Length: 179, dtype: float64
Protein Volume Inside Void'
0 2.505415
1 3.659348
2 1.100627
3 0.918757
4 0.021950
...
1014 0.122292
1015 0.003136
1016 0.006271
1017 0.025086
1018 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1019, dtype: float64
Cell Volume
0 1291.282774
1 2210.233902
2 1197.945872
3 2787.068877
4 2648.076351
5 2145.786097
6 1414.242525
7 1363.017919
8 924.692573
9 826.009322
10 223.781542
11 2047.341158
12 1691.791717
13 1449.415542
14 1675.551986
15 65.137656
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 74678.853665
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
137 MS150-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(46, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.034493
1 0.084664
2 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 1.777935
1 0.084664
2 6.227477
3 0.410775
4 0.624002
...
61 0.012543
62 0.053307
63 0.457811
64 0.235177
65 0.034493
Name: Proten_Volume_Voxel_Resolved, Length: 66, dtype: float64
Cell Volume
0 1466.884463
1 804.090860
2 787.292977
3 331.156921
4 374.269502
5 2616.296149
6 917.972793
7 658.246855
8 2061.815496
9 369.352742
10 2604.251969
11 1184.239777
12 1185.541088
13 2224.833667
14 2507.559880
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 131154.546726
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
140 MS152-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(35, 1024, 1024)
Number of unique cells:
[0 2 3 4 5]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.047035
2 0.006271
3 0.040764
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.119156
1 0.003136
2 0.025086
3 0.006271
4 0.043900
...
558 0.003136
559 0.003136
560 0.003136
561 0.012543
562 0.050171
Name: Proten_Volume_Voxel_Resolved, Length: 563, dtype: float64
Cell Volume
0 1922.515672
1 1805.400845
2 565.972951
3 2023.491112
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 108762.887143
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
143 MS152-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(45, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.009407 1 0.047035 2 0.056442 3 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.068985 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1429.798675 1 343.470770 2 3155.653376 3 1470.302363 4 66.018785 5 624.497440 6 204.613078 7 174.325464 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 140491.664266 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 146 MS156-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.125428
1 0.235177
2 0.003136
3 0.169327
4 0.015678
5 0.454675
6 0.003136
7 0.006271
8 0.003136
9 0.059578
10 0.003136
11 0.043900
12 0.009407
13 0.012543
14 0.131699
15 0.031357
16 0.028221
17 0.003136
18 0.003136
19 0.043900
20 0.003136
21 0.009407
22 0.012543
23 0.006271
24 0.043900
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 5.973486
1 0.291619
2 0.015678
3 0.247719
4 0.238312
...
1186 0.012543
1187 0.003136
1188 0.050171
1189 0.031357
1190 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 1191, dtype: float64
Cell Volume
0 1531.633293
1 626.149948
2 1305.173874
3 1463.124772
4 798.675526
5 695.307556
6 168.941486
7 881.398123
8 954.092788
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 57335.655618
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
149 MS156-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.028221
1 0.021950
2 0.009407
3 0.009407
4 0.012543
5 0.166191
6 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.906214
1 0.272805
2 1.940991
3 0.191277
4 2.464651
...
778 0.003136
779 0.040764
780 0.006271
781 0.003136
782 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 783, dtype: float64
Cell Volume
0 1705.278313
1 651.295033
2 377.668588
3 398.420574
4 405.428838
5 264.275822
6 481.146307
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 77916.677756
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
152 MS157-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(32, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.003136
2 0.025086
3 0.003136
4 0.003136
5 0.003136
6 0.009407
7 0.087799
8 0.006271
9 0.043900
10 0.003136
11 0.050171
12 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.134835
1 0.090935
2 0.062714
3 5.656782
4 0.492303
...
978 0.012543
979 0.131699
980 0.059578
981 0.003136
982 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 983, dtype: float64
Cell Volume
0 77.865416
1 1153.676222
2 2192.313442
3 276.530093
4 2238.966215
5 336.616155
6 1381.270761
7 1909.452394
8 1681.738699
9 379.264653
10 170.158134
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 93418.392591
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
155 MS157-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(35, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.031357
1 0.021950
2 0.003136
3 0.003136
4 0.021950
5 0.003136
6 0.040764
7 0.003136
8 0.015678
9 0.031357
10 0.006271
11 0.003136
12 0.025086
13 0.012543
14 0.065849
15 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 9.846062
1 0.109749
2 9.836655
3 0.859179
4 0.852907
...
973 0.003136
974 0.003136
975 0.018814
976 0.050171
977 0.034493
Name: Proten_Volume_Voxel_Resolved, Length: 978, dtype: float64
Cell Volume
0 1236.787644
1 1159.518010
2 345.966778
3 1967.277625
4 1818.464123
5 1828.554768
6 424.901463
7 43.134530
8 232.194594
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 106023.468188
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
158 MS158-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(41, 1024, 1024)
Number of unique cells:
[0 2 3 4 5]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
2 0.018814
3 0.003136
4 0.028221
5 0.003136
6 0.003136
7 0.003136
8 0.031357
9 0.003136
10 0.003136
11 0.003136
12 0.009407
13 0.003136
14 0.003136
15 0.106613
16 0.003136
17 0.003136
18 0.009407
19 0.006271
20 0.006271
21 0.015678
22 0.003136
23 0.003136
24 0.006271
25 0.003136
26 0.003136
27 0.003136
28 0.009407
29 0.003136
30 0.003136
31 0.006271
32 0.003136
33 0.003136
34 0.003136
35 0.037628
36 0.006271
37 0.003136
38 0.012543
39 0.003136
40 0.003136
41 0.003136
42 0.003136
43 0.003136
44 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 4.487170
1 0.021950
2 6.735459
3 0.025086
4 0.053307
...
2061 0.003136
2062 0.003136
2063 0.003136
2064 0.003136
2065 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 2066, dtype: float64
Cell Volume
0 3034.829028
1 2158.529535
2 1653.404619
3 603.334678
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 127358.215758
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
161 MS158-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(13, 1024, 1024)
Number of unique cells:
[ 0 3 4 5 6 8 9 10 11 12 13 14 15 16]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.012543 1 0.006271 2 0.003136 3 0.003136 4 0.043900 5 0.012543 6 0.003136 7 0.586374 8 0.003136 9 0.003136 10 0.021950 11 0.015678 12 0.003136 13 0.025086 14 0.003136 15 0.003136 16 0.003136 17 0.003136 18 0.003136 19 0.003136 20 0.003136 21 0.163056 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 645.381125 1 188.451740 2 686.997982 3 41.112011 4 446.434236 5 151.002213 6 872.100807 7 178.204310 8 783.793549 9 51.020787 10 457.318210 11 264.576848 12 392.271489 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 37585.434134 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 165 MS160-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.018814
2 2.191846
3 0.103478
4 0.003136
5 0.059578
6 0.009407
7 0.021950
8 0.486032
9 0.009407
10 0.015678
11 0.257126
12 0.423318
13 0.006271
14 0.006271
15 0.018814
16 0.003136
17 0.003136
18 0.003136
19 0.003136
20 0.006271
21 0.025086
22 0.003136
23 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.341790
1 0.275941
2 0.445268
3 0.153649
4 0.150513
...
606 0.031357
607 0.410775
608 0.188141
609 0.228905
610 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 611, dtype: float64
Cell Volume
0 951.756700
1 598.722081
2 742.753658
3 1863.433031
4 809.578315
5 1533.862768
6 686.151346
7 1000.607591
8 526.344120
9 455.809944
10 911.898964
11 1048.916007
12 259.732210
13 90.223164
14 736.077777
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 69984.323555
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
169 MS160-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(14, 1024, 1024)
Number of unique cells:
[0 2 3]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.025086
1 0.103478
2 0.003136
3 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.015678
1 1.232326
2 0.028221
3 0.028221
4 0.448403
...
433 0.003136
434 0.006271
435 0.056442
436 0.015678
437 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 438, dtype: float64
Cell Volume
0 938.006706
1 1037.172854
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 44056.927529
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
172 MS165-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.040764
1 0.025086
2 0.003136
3 0.025086
4 1.207240
5 0.037628
6 0.003136
7 0.025086
8 0.012543
9 0.156784
10 0.379418
11 0.075257
12 0.047035
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.062714
1 0.006271
2 0.153649
3 0.021950
4 0.084664
...
267 0.003136
268 0.028221
269 0.006271
270 0.003136
271 0.040764
Name: Proten_Volume_Voxel_Resolved, Length: 272, dtype: float64
Cell Volume
0 2507.873449
1 2390.419967
2 1736.456463
3 2046.550965
4 881.833984
5 804.517314
6 77.708631
7 44.780767
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 71710.049692
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
175 MS165-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(28, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.181870
1 0.175599
2 0.062714
3 0.028221
4 0.291619
5 0.112885
6 0.006271
7 0.009407
8 0.003136
9 0.021950
10 0.003136
11 0.266534
12 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.028221
2 0.028221
3 0.028221
4 0.774515
...
224 0.006271
225 0.009407
226 0.003136
227 0.015678
228 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 229, dtype: float64
Cell Volume
0 2151.192024
1 1214.198145
2 209.360511
3 169.164120
4 2017.530168
5 1105.204750
6 1302.875414
7 310.448835
8 1315.587495
9 1105.512048
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 81163.140669
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
178 MS167-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(40, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.068985
1 0.059578
2 0.009407
3 0.012543
4 0.015678
5 0.398232
6 0.206955
7 0.210091
8 0.056442
9 0.028221
10 0.009407
11 0.009407
12 0.003136
13 0.003136
14 0.003136
15 0.015678
16 0.304162
17 0.025086
18 0.028221
19 0.112885
20 0.025086
21 0.015678
22 0.031357
23 0.006271
24 0.003136
25 0.003136
26 0.003136
27 0.015678
28 0.075257
29 0.003136
30 0.003136
31 0.003136
32 0.062714
33 1.251140
34 0.059578
35 0.003136
36 0.003136
37 0.003136
38 0.141106
39 0.216363
40 0.294755
41 0.003136
42 0.181870
43 0.006271
44 0.112885
45 0.570695
46 0.062714
47 0.006271
48 0.084664
49 0.310433
50 0.003136
51 0.003136
52 0.012543
53 0.003136
54 0.003136
55 0.003136
56 0.003136
57 0.003136
58 0.116020
59 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.103478
1 0.144242
2 0.103478
3 0.006271
4 6.923600
...
1447 0.025086
1448 0.025086
1449 0.006271
1450 0.009407
1451 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1452, dtype: float64
Cell Volume
0 146.922682
1 566.512289
2 1242.707824
3 1340.400198
4 1940.787329
5 675.621704
6 2644.222590
7 2617.519067
8 1654.132098
9 222.771850
10 1082.872377
11 1642.793449
12 2101.779845
13 630.734324
14 670.936986
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 112339.591357
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
181 MS167-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.025086
1 0.031357
2 0.849772
3 0.003136
4 0.003136
5 0.012543
6 0.448403
7 0.009407
8 0.310433
9 0.313569
10 0.137970
11 0.200684
12 0.003136
13 0.056442
14 0.056442
15 0.003136
16 0.003136
17 0.006271
18 0.012543
19 0.012543
20 0.009407
21 0.134835
22 0.018814
23 0.078392
24 0.047035
25 0.081528
26 0.006271
27 0.185006
28 0.015678
29 0.006271
30 0.003136
31 0.006271
32 0.398232
33 0.025086
34 0.821550
35 0.021950
36 0.031357
37 0.025086
38 0.003136
39 0.009407
40 0.009407
41 0.752565
42 0.360604
43 0.068985
44 0.250855
45 0.003136
46 0.043900
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.003136
2 0.319840
3 0.075257
4 0.031357
...
1852 0.012543
1853 0.003136
1854 0.012543
1855 0.006271
1856 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 1857, dtype: float64
Cell Volume
0 438.030591
1 1026.502106
2 551.410813
3 2118.157546
4 2950.996398
5 1038.097882
6 813.454026
7 1461.707441
8 552.624325
9 553.834701
10 66.062684
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 90357.358615
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
184 MS170-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 8 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.043900
1 0.037628
2 0.128563
3 0.003136
4 0.009407
5 0.050171
6 0.003136
7 0.003136
8 0.053307
9 0.006271
10 0.003136
11 0.009407
12 0.329247
13 0.003136
14 0.134835
15 0.003136
16 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.257126
1 0.025086
2 0.003136
3 0.009407
4 0.084664
...
693 0.003136
694 0.009407
695 0.034493
696 0.006271
697 0.031357
Name: Proten_Volume_Voxel_Resolved, Length: 698, dtype: float64
Cell Volume
0 1359.342892
1 1531.648972
2 1803.268577
3 403.531746
4 1603.794892
5 346.640951
6 319.956242
7 823.494500
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 90448.550706
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
187 MS170-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.003136
2 0.200684
3 0.025086
4 0.288483
...
64 0.003136
65 2.543043
66 0.517389
67 0.003136
68 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 69, dtype: float64
Protein Volume Inside Void'
0 0.018814
1 0.031357
2 0.994013
3 0.062714
4 0.006271
...
1114 0.090935
1115 0.021950
1116 0.003136
1117 0.009407
1118 0.015678
Name: Proten_Volume_Voxel_Resolved, Length: 1119, dtype: float64
Cell Volume
0 1024.222460
1 2465.428770
2 1309.482310
3 1977.148772
4 1857.625736
5 1368.536730
6 1694.171704
7 2364.531723
8 1272.267959
9 964.368439
10 125.505930
11 1721.916275
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 83783.030317
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
190 MS171-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(41, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.065849
3 0.003136
4 0.031357
...
78 0.003136
79 0.003136
80 0.003136
81 0.003136
82 0.015678
Name: Proten_Volume_Voxel_Resolved, Length: 83, dtype: float64
Cell Volume
0 1551.595086
1 478.948189
2 2423.256896
3 1327.208356
4 3235.707502
5 1187.767427
6 1404.534434
7 40.503688
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 123158.792041
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
193 MS171-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(36, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.056442
1 0.109749
2 0.417047
3 0.116020
4 0.185006
5 0.250855
6 0.050171
7 0.009407
8 0.078392
9 0.040764
10 0.131699
11 0.043900
12 0.003136
13 0.009407
14 0.009407
15 0.034493
16 0.031357
17 0.025086
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.094071
1 0.445268
2 0.144242
3 0.025086
4 0.018814
...
80 0.028221
81 0.003136
82 0.128563
83 0.025086
84 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 85, dtype: float64
Cell Volume
0 532.853809
1 151.569772
2 1092.188507
3 1185.619480
4 1189.880881
5 1148.248345
6 1309.902492
7 1240.973788
8 1459.139312
9 427.350436
10 118.579194
11 1467.574314
12 1385.792424
13 1340.654189
14 970.893807
15 337.917466
16 110.357419
17 340.661193
18 220.937472
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 102337.181071
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
196 MS172-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.021950 1 0.003136 2 0.131699 3 0.006271 4 0.068985 5 0.062714 6 0.003136 7 0.006271 8 0.003136 9 0.003136 10 0.012543 11 0.015678 12 0.003136 13 0.034493 14 0.003136 15 0.009407 16 0.003136 17 0.122292 18 0.047035 19 0.034493 20 0.003136 21 0.003136 22 0.003136 23 0.003136 24 0.003136 25 0.003136 26 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 876.475092 1 2217.483613 2 620.637407 3 571.585833 4 2078.713721 5 1446.383332 6 950.950828 7 131.441788 8 207.557490 9 1324.273352 10 211.649563 11 121.241394 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 87881.836063 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 199 MS172-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (24, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.018814 1 0.015678 2 0.131699 3 0.012543 4 0.134835 5 0.090935 6 0.031357 7 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.040764 1 0.009407 2 0.006271 3 0.006271 4 0.031357 5 0.003136 6 0.018814 7 0.003136 8 0.078392 9 0.003136 10 0.003136 11 0.028221 12 0.003136 13 0.031357 14 0.003136 15 0.003136 16 0.144242 17 0.150513 18 0.009407 19 0.040764 20 0.031357 21 0.172463 22 0.006271 23 0.003136 24 0.119156 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 702.971179 1 629.088088 2 657.450390 3 603.049331 4 751.655878 5 457.010913 6 268.973084 7 1110.209309 8 1692.929971 9 771.601992 10 227.999043 11 997.105027 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 70042.139379 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 202 MS175-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (26, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 13 14 15 16 17 18 19 20 21 22 23 24 25] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.009407
1 0.028221
2 0.006271
3 0.006271
4 0.009407
5 0.131699
6 0.003136
7 0.106613
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.084664
1 0.018814
2 0.015678
3 0.003136
4 0.003136
...
172 0.012543
173 0.006271
174 1.194697
175 0.003136
176 0.363740
Name: Proten_Volume_Voxel_Resolved, Length: 177, dtype: float64
Cell Volume
0 703.686116
1 729.590038
2 169.308362
3 718.665300
4 360.183989
5 155.163271
6 513.544240
7 1236.837815
8 189.028707
9 1293.079523
10 1620.542604
11 1200.172210
12 539.062472
13 984.214211
14 48.891654
15 462.200477
16 82.985995
17 362.962209
18 116.475147
19 108.215744
20 117.161863
21 570.773690
22 451.931097
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 72753.522144
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
205 MS175-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(15, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.003136
1 0.219498
2 0.003136
3 0.006271
4 0.037628
5 0.006271
6 0.012543
7 0.018814
8 0.006271
9 0.034493
10 0.021950
11 0.003136
12 0.006271
13 0.034493
14 0.028221
15 0.341790
16 0.037628
17 0.012543
18 0.335519
19 0.395097
20 0.006271
21 0.003136
22 0.084664
23 0.003136
24 0.012543
25 0.003136
26 0.012543
27 0.037628
28 0.003136
29 0.053307
30 0.006271
31 0.090935
32 0.021950
33 0.435861
34 0.003136
35 0.003136
36 0.034493
37 0.009407
38 0.003136
39 0.003136
40 0.438996
41 0.191277
42 0.037628
43 0.003136
44 0.021950
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.050171
1 0.235177
2 0.489167
3 0.034493
4 0.062714
...
430 0.021950
431 0.003136
432 0.003136
433 0.006271
434 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 435, dtype: float64
Cell Volume
0 509.072748
1 1053.647761
2 1060.430255
3 885.982499
4 900.751592
5 83.243121
6 131.435517
7 506.893445
8 999.055425
9 611.371448
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 42578.230928
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
209 MS178-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.498574
1 0.821550
2 0.003136
3 0.006271
4 0.018814
5 0.015678
6 0.009407
7 0.040764
8 0.009407
9 0.003136
10 0.003136
11 0.003136
12 0.006271
13 0.012543
14 0.003136
15 0.006271
16 0.003136
17 0.003136
18 0.003136
19 0.003136
20 0.003136
21 0.009407
22 0.009407
23 0.003136
24 0.003136
25 0.015678
26 0.003136
27 0.003136
28 0.009407
29 0.003136
30 0.003136
31 0.034493
32 0.009407
33 0.106613
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.460946
2 0.341790
3 0.028221
4 0.009407
...
726 0.003136
727 0.003136
728 0.056442
729 0.006271
730 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 731, dtype: float64
Cell Volume
0 2060.498507
1 468.845001
2 1694.162297
3 1345.724597
4 108.654740
5 230.585986
6 629.940995
7 1425.038700
8 474.752638
9 525.033402
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 89676.992614
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
213 MS178-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.009407
2 0.003136
3 0.072121
4 0.006271
5 0.003136
6 0.003136
7 0.003136
8 0.003136
9 0.040764
10 0.015678
11 0.003136
12 0.006271
13 3.176452
14 0.006271
15 0.003136
16 0.006271
17 0.156784
18 0.031357
19 0.003136
20 0.150513
21 0.015678
22 0.006271
23 0.370011
24 0.200684
25 0.006271
26 0.015678
27 1.106898
28 0.003136
29 0.003136
30 0.003136
31 0.003136
32 0.006271
33 0.103478
34 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.301026
1 0.059578
2 0.009407
3 0.003136
4 0.003136
...
1446 0.003136
1447 0.021950
1448 0.003136
1449 0.003136
1450 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1451, dtype: float64
Cell Volume
0 529.755749
1 817.530421
2 1315.791315
3 1500.552350
4 1294.537619
5 724.635650
6 641.781354
7 106.851720
8 521.113792
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 58307.603016
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
217 MS180-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.150513 1 0.006271 2 1.539623 3 0.012543 4 0.043900 5 0.460946 6 0.012543 7 0.040764 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.122292 1 0.119156 2 0.037628 3 0.200684 4 0.100342 5 0.210091 6 0.075257 7 0.003136 8 0.050171 9 0.018814 10 0.219498 11 0.238312 12 0.003136 13 0.771379 14 0.003136 15 0.285348 16 0.244584 17 0.025086 18 0.047035 19 0.031357 20 0.090935 21 0.090935 22 0.009407 23 0.172463 24 0.736887 25 0.009407 26 0.006271 27 0.075257 28 0.028221 29 0.175599 30 0.116020 31 0.003136 32 0.006271 33 0.037628 34 0.150513 35 0.037628 36 0.028221 37 0.003136 38 0.003136 39 0.003136 40 0.003136 41 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 854.544087 1 957.469925 2 911.604210 3 1000.228172 4 127.302680 5 370.265228 6 662.354606 7 783.683799 8 464.621229 9 358.867000 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 59269.212049 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 221 MS180-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.012543 2 0.006271 3 0.003136 4 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.834093 1 0.034493 2 0.047035 3 0.068985 4 0.006271 5 0.018814 6 0.003136 7 0.075257 8 0.012543 9 0.181870 10 0.012543 11 0.006271 12 0.235177 13 0.153649 14 0.097206 15 0.006271 16 0.370011 17 0.075257 18 0.003136 19 0.006271 20 0.003136 21 0.012543 22 0.003136 23 0.003136 24 0.081528 25 0.003136 26 0.003136 27 0.147377 28 0.238312 29 0.034493 30 0.028221 31 0.003136 32 0.404504 33 0.094071 34 0.065849 35 0.090935 36 0.006271 37 0.003136 38 0.043900 39 0.028221 40 0.366876 41 0.009407 42 0.727480 43 0.003136 44 0.203820 45 0.056442 46 0.003136 47 0.015678 48 0.021950 49 0.056442 50 0.003136 51 0.003136 52 0.006271 53 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1854.101222 1 960.085089 2 1577.326545 3 2042.785003 4 1934.387389 5 225.521849 6 1176.256315 7 155.479976 8 245.841110 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 72028.406733 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 225 MS187-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14] VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.150513
1 0.037628
2 0.037628
3 0.225770
4 0.025086
5 0.012543
6 0.012543
7 0.037628
8 0.012543
9 0.112885
10 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.025086
2 0.012543
3 0.025086
4 0.012543
...
84 0.062714
85 0.025086
86 0.012543
87 0.012543
88 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 89, dtype: float64
Cell Volume
0 788.675815
1 1046.579919
2 594.162790
3 1083.066790
4 1075.327911
5 1135.119218
6 1683.212473
7 458.851562
8 1163.854667
9 528.827585
10 671.275640
11 899.102220
12 427.181109
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 317245.527226
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
228 MS187-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(41, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.137970
2 0.075257
3 0.050171
4 0.112885
5 0.012543
6 0.037628
7 0.012543
8 0.012543
9 0.163056
10 0.025086
11 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.050171
2 0.025086
3 0.037628
4 0.012543
...
152 0.802736
153 0.025086
154 0.112885
155 0.012543
156 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 157, dtype: float64
Cell Volume
0 1881.174756
1 2659.126517
2 1802.117779
3 1420.103127
4 439.121810
5 324.029501
6 1694.187383
7 3122.117188
8 845.946029
9 1232.388274
10 2093.611377
11 1305.612870
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 520413.717864
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
231 MS188-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(10, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=1.0)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.031357
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.041809
1 1.191562
2 0.449449
3 0.041809
4 0.010452
...
165 0.010452
166 0.010452
167 0.010452
168 0.020905
169 0.010452
Name: Proten_Volume_Voxel_Resolved, Length: 170, dtype: float64
Cell Volume
0 1739.397739
1 2710.834020
2 1563.339285
3 178.002581
4 2309.507707
5 2081.010090
6 2048.294407
7 1368.487604
8 332.518855
9 1330.221753
10 1494.887206
11 1012.231588
12 573.465156
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 90858.056984
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
234 MS188-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(11, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=1.0)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.062714
1 0.114975
2 0.010452
3 0.020905
4 0.010452
5 0.010452
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 1.431964
1 0.010452
2 0.501710
3 0.010452
4 0.010452
...
296 0.010452
297 0.010452
298 0.010452
299 0.010452
300 0.083618
Name: Proten_Volume_Voxel_Resolved, Length: 301, dtype: float64
Cell Volume
0 1442.594375
1 122.124613
2 280.435070
3 1281.681296
4 1110.807180
5 1701.236410
6 1293.032488
7 147.617760
8 626.907739
9 1812.124806
10 556.825102
11 1778.123491
12 326.132503
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 108080.637639
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
237 MS190-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(24, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.025086
1 0.003136
2 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.009407
2 0.003136
3 0.003136
4 0.003136
...
607 0.028221
608 0.003136
609 0.003136
610 0.006271
611 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 612, dtype: float64
Cell Volume
0 1339.911030
1 846.617067
2 1041.829351
3 94.497107
4 352.768086
5 351.124985
6 998.616428
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 73886.819527
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
240 MS190-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.006271
2 0.006271
3 0.003136
4 0.003136
5 0.003136
6 0.003136
7 0.006271
8 0.031357
9 0.006271
10 0.006271
11 0.003136
12 0.009407
13 0.003136
14 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.144242
1 0.072121
2 0.059578
3 0.003136
4 0.137970
...
1220 0.003136
1221 0.003136
1222 0.003136
1223 0.006271
1224 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1225, dtype: float64
Cell Volume
0 228.444310
1 1205.161091
2 858.046651
3 758.193788
4 1477.592839
5 749.141056
6 1051.907454
7 186.949745
8 1614.688273
9 1006.377257
10 1336.392788
11 1229.330977
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 63921.949703
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
243 MS193-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.003136 1 0.103478 2 0.134835 3 0.003136 4 0.385690 5 0.003136 6 0.034493 7 0.065849 8 0.003136 9 0.015678 10 0.006271 11 0.925028 12 0.003136 13 0.012543 14 0.003136 15 0.009407 16 0.003136 17 0.025086 18 0.062714 19 0.062714 20 0.319840 21 0.003136 22 0.504846 23 0.360604 24 0.012543 25 0.021950 26 0.021950 27 0.078392 28 0.624002 29 0.078392 30 0.009407 31 0.137970 32 0.006271 33 0.006271 34 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1096.315073 1 916.753010 2 1264.942991 3 619.991455 4 1053.067658 5 1461.688627 6 549.519993 7 255.985062 8 1260.292765 9 1038.957060 10 916.862759 11 604.244028 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 54721.532501 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 246 MS193-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (14, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.015678
2 0.003136
3 0.018814
4 0.012543
5 0.003136
6 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.028221
2 0.003136
3 0.028221
4 0.065849
...
97 0.012543
98 0.006271
99 0.003136
100 0.025086
101 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 102, dtype: float64
Cell Volume
0 933.579114
1 596.226073
2 237.224238
3 920.754149
4 940.041768
5 790.946054
6 266.787509
7 625.751715
8 757.105704
9 543.508879
10 1102.674250
11 91.869400
12 97.704916
13 947.209952
14 363.260100
15 605.764837
16 655.437278
17 323.543470
18 828.546094
19 374.975032
20 219.535820
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 33809.66074
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
249 MS195-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(38, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.056442
1 0.003136
2 0.006271
3 0.015678
4 0.003136
5 0.003136
6 0.015678
7 0.003136
8 0.003136
9 0.018814
10 0.009407
11 0.128563
12 0.003136
13 0.006271
14 0.006271
15 0.012543
16 0.006271
17 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.006271
2 0.025086
3 0.009407
4 0.003136
...
380 0.003136
381 0.006271
382 0.003136
383 0.003136
384 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 385, dtype: float64
Cell Volume
0 1364.068374
1 744.334045
2 382.974173
3 2374.776017
4 2786.526403
5 240.309756
6 1469.374199
7 250.453708
8 1649.883240
9 903.338535
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 112778.252221
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
252 MS195-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.216363
1 0.147377
2 0.009407
3 0.021950
4 0.003136
5 0.047035
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.003136
2 0.103478
3 0.003136
4 0.122292
...
275 0.009407
276 0.006271
277 0.003136
278 0.003136
279 0.018814
Name: Proten_Volume_Voxel_Resolved, Length: 280, dtype: float64
Cell Volume
0 1694.946219
1 1366.874815
2 547.945878
3 707.207494
4 458.186796
5 954.070838
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 76470.95919
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
255 MS197-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.103478 1 0.003136 2 0.047035 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2805.356212 1 2383.910278 2 1512.910098 3 548.761157 4 1021.898915 5 467.352413 6 231.351094 7 356.439977 8 600.233483 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 55831.939359 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 258 MS197-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.059578 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.018814 1 0.116020 2 0.081528 3 0.012543 4 0.003136 5 0.006271 6 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1867.070429 1 1302.734308 2 1486.241067 3 638.683294 4 1071.684241 5 1380.408447 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 48149.30825 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 261 MS199-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.194413 1 0.094071 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.275941 1 0.009407 2 0.015678 3 0.006271 4 0.012543 5 0.003136 6 0.090935 7 0.028221 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 772.088024 1 507.172521 2 965.487880 3 737.529601 4 437.604137 5 599.067006 6 1111.469856 7 223.856798 8 897.891844 9 300.182591 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 49343.779779 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 264 MS199-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.012543 1 0.037628 2 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.040764 1 1.740307 2 0.150513 3 0.326112 4 0.009407 5 0.188141 6 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1096.691356 1 302.151803 2 1008.823094 3 368.957645 4 353.338781 5 1528.343956 6 316.829961 7 536.390866 8 429.144050 9 531.185623 10 488.891457 11 554.295647 12 326.572545 13 264.050053 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 47790.4632 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 267 MS200-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.511117
1 0.072121
2 0.150513
3 0.125428
4 0.072121
5 0.166191
6 0.012543
7 0.068985
8 0.062714
9 0.037628
10 0.012543
11 0.034493
12 0.047035
13 0.015678
14 0.028221
15 0.034493
16 0.075257
17 0.031357
18 0.018814
19 0.012543
20 0.006271
21 0.040764
22 0.109749
23 0.015678
24 0.018814
25 0.003136
26 0.003136
27 0.065849
28 0.006271
29 0.009407
30 0.009407
31 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.987742
1 0.388825
2 0.639680
3 0.357468
4 0.536203
...
693 0.012543
694 0.084664
695 0.172463
696 0.166191
697 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 698, dtype: float64
Cell Volume
0 1153.174512
1 1003.743279
2 693.905904
3 1405.776166
4 155.671253
5 940.584242
6 884.740767
7 823.566621
8 754.644189
9 309.291766
10 257.998174
11 223.609079
12 1174.453294
13 636.466363
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 71782.565623
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
270 MS200-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(18, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.144242
1 0.068985
2 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.445268
1 0.131699
2 0.006271
3 0.009407
4 0.163056
...
255 0.006271
256 0.018814
257 0.028221
258 0.006271
259 0.084664
Name: Proten_Volume_Voxel_Resolved, Length: 260, dtype: float64
Cell Volume
0 442.690224
1 1766.160839
2 1587.022094
3 335.060853
4 1005.772069
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 54047.431606
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
273 MS201-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(22, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 17 18 19 20 21 22 23 24]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.081528 1 0.075257 2 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1247.539920 1 982.003551 2 891.482497 3 1235.580404 4 1296.368861 5 1729.532863 6 1239.130004 7 188.451740 8 1161.054497 9 730.066663 10 226.650697 11 569.992903 12 851.668661 13 900.055469 14 46.091484 15 192.045239 16 1698.028601 17 319.184863 18 551.583276 19 499.944759 20 364.680566 21 111.395332 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 55303.635433 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 276 MS201-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (26, 1024, 1024) Number of unique cells: [0 2 3 4 5 6] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.266534 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.094071 1 0.078392 2 0.937571 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1858.538221 1 2691.019604 2 1676.238702 3 749.332333 4 1857.484630 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 76655.585391 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 279 MS203-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 1.147662 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.018814 1 0.094071 2 0.003136 3 0.103478 4 0.090935 5 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2095.304649 1 1009.550574 2 349.786046 3 664.555860 4 346.405774 5 1243.617174 6 275.517265 7 363.918594 8 1165.052500 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 58246.444549 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 282 MS203-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [0 2 3 4 5] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 1.248004 1 0.018814 2 0.003136 3 0.451539 4 0.090935 5 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2030.731417 1 2361.154587 2 1225.806463 3 2978.637491 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 73603.861273 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 284 MS204-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
Protein Volume Inside Cells'
0 0.012543
1 0.050171
2 0.025086
3 0.012543
4 0.075257
5 0.025086
6 0.012543
7 0.012543
8 0.087799
9 0.012543
10 0.012543
11 0.012543
12 0.100342
13 0.012543
14 0.037628
15 0.012543
16 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.037628
1 0.012543
2 0.012543
3 0.852907
4 0.062714
...
334 0.012543
335 0.062714
336 0.012543
337 0.037628
338 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 339, dtype: float64
Cell Volume
0 475.395454
1 500.493504
2 2005.874814
3 1369.355145
4 1391.881931
5 1506.046076
6 1167.730378
7 739.859418
8 2170.034376
9 1336.706357
10 906.502445
11 538.059052
12 1536.462254
13 538.121766
14 218.607656
15 1370.170424
16 1099.384912
17 1575.871586
18 1669.628671
19 441.956473
20 629.897095
21 291.192572
22 907.066869
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 199198.220923
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
288 MS204-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(11, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.204472930473117, y=0.204472930473117, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.087799
1 0.025086
2 0.025086
3 0.012543
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.012543
2 0.037628
3 0.062714
4 0.087799
...
319 0.012543
320 0.012543
321 0.012543
322 0.012543
323 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 324, dtype: float64
Cell Volume
0 822.917533
1 799.073758
2 1243.839808
3 440.927967
4 1161.622057
5 1019.098745
6 639.103477
7 1686.285448
8 874.443166
9 836.714563
10 566.832129
11 634.425029
12 1326.120273
13 853.521853
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 131767.410762
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
292 MS207-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(16, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.119156 1 0.106613 2 0.178734 3 0.031357 4 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.144242 1 0.247719 2 0.150513 3 0.021950 4 0.084664 5 0.065849 6 0.065849 7 0.003136 8 0.003136 9 0.021950 10 0.003136 11 0.166191 12 0.028221 13 0.018814 14 0.015678 15 0.028221 16 0.028221 17 0.012543 18 0.003136 19 0.028221 20 0.015678 21 0.078392 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 508.386032 1 213.461991 2 741.496247 3 556.064175 4 1288.137678 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 49300.576264 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 295 MS207-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.062714 1 0.228905 2 0.006271 3 0.116020 4 0.050171 5 0.137970 6 0.065849 7 0.304162 8 0.003136 9 0.003136 10 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.188141 2 0.122292 3 0.015678 4 0.003136 5 0.062714 6 0.003136 7 0.056442 8 0.250855 9 0.037628 10 0.059578 11 0.896807 12 0.028221 13 0.031357 14 0.025086 15 0.031357 16 0.006271 17 0.018814 18 0.025086 19 0.012543 20 0.021950 21 0.122292 22 0.109749 23 0.094071 24 0.172463 25 0.072121 26 0.012543 27 0.197548 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2284.919729 1 606.859192 2 1425.283284 3 586.154241 4 1439.745079 5 1289.260255 6 790.325188 7 361.905482 8 658.955520 9 399.853584 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 46052.868482 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 299 MS230-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.015678
2 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.009407
2 0.297890
3 0.003136
4 0.003136
...
274 0.006271
275 0.053307
276 0.009407
277 0.006271
278 0.018814
Name: Proten_Volume_Voxel_Resolved, Length: 279, dtype: float64
Cell Volume
0 1505.654115
1 788.421825
2 1441.488522
3 1264.021099
4 1648.531759
5 527.215841
6 285.893258
7 1409.065503
8 886.989055
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 56002.872007
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
302 MS230-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(21, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.003136
2 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.003136
2 0.153649
3 0.548745
4 0.250855
...
296 0.006271
297 0.003136
298 0.144242
299 0.028221
300 0.072121
Name: Proten_Volume_Voxel_Resolved, Length: 301, dtype: float64
Cell Volume
0 1474.256466
1 629.282500
2 1249.443283
3 208.611081
4 345.392947
5 1795.291385
6 837.391871
7 1050.164011
8 62.839196
9 1106.433940
10 191.371066
11 198.463993
12 512.258607
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 59386.960286
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
305 MS234-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(19, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.034493 1 0.006271 2 0.253991 3 0.006271 4 0.043900 5 0.003136 6 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.056442 1 0.006271 2 0.043900 3 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1209.598090 1 971.687136 2 1162.465557 3 1087.074200 4 119.149890 5 296.971646 6 713.632520 7 304.287207 8 259.631868 9 468.035993 10 1018.032611 11 134.119666 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 54727.458953 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 309 MS234-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.159920 1 0.134835 2 0.018814 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.006271 1 0.116020 2 1.837513 3 0.047035 4 0.009407 5 0.034493 6 0.047035 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1078.774032 1 373.880676 2 1062.543709 3 1934.541038 4 1375.174983 5 1530.331983 6 742.076349 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 47798.807267 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 313 MS238-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (24, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.053307 1 0.015678 2 0.025086 3 0.166191 4 0.194413 5 0.003136 6 0.018814 7 0.003136 8 0.153649 9 0.009407 10 0.047035 11 0.028221 12 0.009407 13 0.012543 14 0.106613 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.097206 1 0.006271 2 0.006271 3 0.050171 4 0.009407 5 0.003136 6 0.053307 7 0.043900 8 0.103478 9 0.003136 10 0.012543 11 0.018814 12 0.141106 13 0.504846 14 0.062714 15 0.037628 16 0.003136 17 0.116020 18 0.006271 19 0.893671 20 0.285348 21 0.097206 22 1.119441 23 0.043900 24 0.006271 25 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1465.307211 1 1580.042052 2 880.488773 3 1397.629648 4 421.408306 5 782.181805 6 1223.624024 7 687.358586 8 1304.578093 9 1256.561296 10 79.433260 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 67833.570528 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 316 MS238-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (24, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.012543 1 0.018814 2 0.018814 3 0.021950 4 0.141106 5 0.015678 6 0.075257 7 0.009407 8 0.028221 9 0.003136 10 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.332383 1 0.081528 2 0.065849 3 0.015678 4 0.003136 5 0.050171 6 0.081528 7 0.062714 8 0.021950 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1284.321546 1 1419.444632 2 1026.868981 3 775.195491 4 863.872760 5 758.055818 6 302.869876 7 767.171264 8 1350.070661 9 169.530996 10 1588.132128 11 230.168939 12 1063.559672 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 67312.920818 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 320 MS239-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (30, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.059578
1 0.009407
2 0.141106
3 0.003136
4 0.003136
...
65 0.015678
66 0.009407
67 0.006271
68 0.081528
69 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 70, dtype: float64
Protein Volume Inside Void'
0 0.037628
1 0.059578
2 0.285348
3 0.141106
4 0.003136
...
3573 0.015678
3574 0.009407
3575 0.006271
3576 0.003136
3577 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 3578, dtype: float64
Cell Volume
0 399.238989
1 498.317337
2 1247.006853
3 474.680517
4 382.083637
5 454.571347
6 1079.811945
7 825.096837
8 754.107986
9 64.582639
10 46.044449
11 372.300289
12 141.127930
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 91901.258721
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
323 MS239-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(35, 1024, 1024)
Number of unique cells:
[0 2 3 4 5]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.100342
2 0.003136
3 0.009407
4 0.003136
...
60 0.003136
61 0.003136
62 0.003136
63 0.003136
64 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 65, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.012543
3 0.094071
4 1.037913
...
7264 0.003136
7265 0.006271
7266 0.009407
7267 0.003136
7268 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 7269, dtype: float64
Cell Volume
0 549.372616
1 2766.194599
2 2435.106663
3 1307.654203
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 108021.939642
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
326 MS242-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.003136 2 0.043900 3 0.053307 4 0.003136 5 0.081528 6 0.021950 7 0.006271 8 0.003136 9 0.059578 10 0.225770 11 0.012543 12 0.081528 13 0.003136 14 0.037628 15 0.012543 16 0.106613 17 0.047035 18 0.006271 19 0.015678 20 0.003136 21 0.009407 22 0.232041 23 0.116020 24 0.018814 25 0.078392 26 0.003136 27 0.006271 28 0.015678 29 0.003136 30 0.015678 31 0.031357 32 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.003136 2 0.391961 3 0.031357 4 0.068985 5 0.037628 6 0.006271 7 0.018814 8 0.003136 9 0.006271 10 0.025086 11 0.078392 12 0.006271 13 0.012543 14 0.006271 15 0.025086 16 0.037628 17 0.025086 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 496.545673 1 422.925979 2 940.076261 3 647.356609 4 355.060274 5 1209.409949 6 1170.010023 7 626.802171 8 805.125637 9 1989.726019 10 826.871636 11 214.017008 12 610.026238 13 1001.065401 14 93.531315 15 499.882045 16 3041.881192 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 86977.923697 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 329 MS242-9_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (33, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.047035 1 0.166191 2 0.084664 3 0.025086 4 0.003136 5 0.009407 6 0.012543 7 0.015678 8 0.015678 9 0.009407 10 0.213227 11 0.081528 12 0.021950 13 0.028221 14 0.028221 15 0.043900 16 0.028221 17 0.021950 18 0.006271 19 0.034493 20 0.012543 21 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.015678 1 0.003136 2 0.012543 3 0.053307 4 0.006271 5 0.159920 6 0.009407 7 0.021950 8 0.003136 9 0.021950 10 0.081528 11 0.003136 12 0.003136 13 0.097206 14 0.003136 15 0.034493 16 0.037628 17 0.015678 18 0.078392 19 0.015678 20 0.072121 21 0.009407 22 0.003136 23 0.050171 24 0.131699 25 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1185.077006 1 1458.443189 2 237.760441 3 1019.569099 4 2062.693489 5 1586.852767 6 53.930706 7 1214.057039 8 347.609879 9 302.819705 10 1580.437148 11 1478.687194 12 1370.280173 13 133.708891 14 172.801519 15 1272.744584 16 131.626794 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 92895.152803 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 332 MS243-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (16, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.003136
1 0.021950
2 0.003136
3 0.003136
4 0.003136
5 0.702394
6 0.003136
7 0.050171
8 0.006271
9 0.003136
10 0.282212
11 0.529931
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 3.568413
1 1.442417
2 0.664766
3 27.198962
4 0.790193
...
639 0.009407
640 0.015678
641 0.003136
642 0.003136
643 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 644, dtype: float64
Cell Volume
0 1160.270575
1 852.173507
2 981.586504
3 156.768744
4 1921.480895
5 122.702625
6 828.480245
7 407.978152
8 189.370497
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 45987.310644
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
335 MS243-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(22, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.040764
1 0.028221
2 0.015678
3 0.003136
4 0.191277
5 0.015678
6 0.489167
7 0.003136
8 0.003136
9 0.009407
10 0.103478
11 0.087799
12 0.006271
13 0.012543
14 0.003136
15 0.003136
16 0.015678
17 0.188141
18 0.040764
19 0.025086
20 0.003136
21 0.043900
22 0.003136
23 0.034493
24 0.009407
25 0.021950
26 0.003136
27 0.006271
28 0.009407
29 0.034493
30 0.003136
31 0.003136
32 0.006271
33 0.003136
34 0.003136
35 0.119156
36 0.003136
37 0.059578
38 0.009407
39 0.031357
40 0.053307
41 0.012543
42 0.065849
43 0.031357
44 0.003136
45 0.043900
46 0.003136
47 0.003136
48 0.106613
49 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.047035
2 0.144242
3 0.018814
4 0.028221
...
1182 0.003136
1183 0.018814
1184 0.003136
1185 0.015678
1186 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1187, dtype: float64
Cell Volume
0 921.472221
1 151.027298
2 116.876515
3 70.189250
4 1282.791330
5 1251.199269
6 803.203460
7 191.085718
8 1284.967497
9 1039.016638
10 377.226456
11 1323.003398
12 69.358293
13 75.325508
14 283.942860
15 1449.334014
16 1624.810276
17 58.828651
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 59962.509629
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
339 MS244-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.084664
1 0.147377
2 0.432725
3 0.153649
4 0.388825
...
79 0.627138
80 0.131699
81 0.050171
82 0.015678
83 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 84, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.047035
2 0.250855
3 0.370011
4 1.953534
...
679 0.012543
680 0.006271
681 0.003136
682 0.003136
683 0.028221
Name: Proten_Volume_Voxel_Resolved, Length: 684, dtype: float64
Cell Volume
0 1893.196986
1 1501.386443
2 514.165106
3 916.197993
4 1825.441030
5 784.157288
6 919.731914
7 721.120544
8 235.408674
9 1351.353158
10 2009.916717
11 275.231918
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 52812.845215
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
342 MS244-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.068985
1 0.009407
2 0.009407
3 0.003136
4 0.238312
5 0.009407
6 0.109749
7 0.015678
8 0.003136
9 0.003136
10 0.006271
11 0.081528
12 0.003136
13 0.009407
14 0.018814
15 0.009407
16 0.103478
17 0.009407
18 0.003136
19 0.072121
20 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.203820
1 0.040764
2 0.003136
3 0.003136
4 0.003136
...
1028 0.003136
1029 0.072121
1030 0.003136
1031 0.003136
1032 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1033, dtype: float64
Cell Volume
0 1864.069576
1 1454.344845
2 1948.852320
3 2029.643333
4 1855.882293
5 937.715087
6 237.537807
7 166.257337
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 71705.888634
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
345 MS246-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(22, 1024, 1024)
Number of unique cells:
[0 2 3 4 6 7]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.507982
1 0.322976
2 0.457811
3 0.043900
4 0.015678
5 0.003136
6 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.134835
2 0.050171
3 0.125428
4 0.918757
...
261 0.003136
262 0.034493
263 0.003136
264 0.003136
265 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 266, dtype: float64
Cell Volume
0 1717.808524
1 2631.137362
2 1818.598957
3 834.877049
4 523.456151
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 64810.29024
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
348 MS246-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(17, 968, 968)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 12 13 14 15 16 17]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.015678 2 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.021950 1 0.395097 2 0.486032 3 0.053307 4 0.003136 5 0.009407 6 0.006271 7 0.003136 8 0.163056 9 0.034493 10 0.012543 11 0.094071 12 0.003136 13 0.003136 14 0.191277 15 0.003136 16 0.178734 17 0.031357 18 0.301026 19 0.178734 20 0.112885 21 0.003136 22 0.442132 23 0.003136 24 0.003136 25 0.003136 26 0.003136 27 0.178734 28 0.090935 29 0.006271 30 0.294755 31 0.100342 32 0.006271 33 0.003136 34 0.003136 35 0.003136 36 0.244584 37 0.109749 38 0.003136 39 0.028221 40 0.006271 41 0.006271 42 0.078392 43 0.003136 44 0.012543 45 0.003136 46 0.012543 47 0.003136 48 0.003136 49 0.003136 50 0.003136 51 0.003136 52 0.009407 53 0.006271 54 0.006271 55 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 819.107672 1 560.610923 2 1139.067050 3 621.722355 4 1323.091198 5 728.103722 6 4.684719 7 184.215425 8 152.902440 9 401.459056 10 984.446252 11 1076.478708 12 494.090429 13 316.986745 14 196.425796 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 40946.268147 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 351 MS248-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (41, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.062714
1 0.003136
2 0.003136
3 0.003136
4 0.006271
5 0.006271
6 0.003136
7 0.006271
8 0.040764
9 0.034493
10 0.006271
11 0.009407
12 0.037628
13 0.015678
14 0.031357
15 0.003136
16 0.009407
17 0.043900
18 0.012543
19 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.084664
1 0.028221
2 0.006271
3 0.040764
4 0.003136
...
1188 0.006271
1189 0.003136
1190 0.003136
1191 0.003136
1192 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1193, dtype: float64
Cell Volume
0 957.153220
1 1083.292559
2 1197.168221
3 659.764528
4 336.926588
5 68.953789
6 819.458869
7 148.020173
8 349.876981
9 156.078892
10 501.164542
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 128530.455256
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
354 MS248-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.021950 1 0.012543 2 0.009407 3 0.040764 4 0.056442 5 0.006271 6 0.006271 7 0.109749 8 0.056442 9 0.034493 10 0.028221 11 0.003136 12 0.003136 13 0.009407 14 0.009407 15 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.009407 1 0.043900 2 0.003136 3 0.112885 4 0.012543 5 0.084664 6 0.015678 7 0.040764 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1714.152311 1 1396.199774 2 1388.642765 3 1009.566252 4 1252.372016 5 695.627397 6 842.509315 7 1487.971967 8 1143.974402 9 642.204672 10 1510.708844 11 779.576048 12 1343.557836 13 414.120966 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 86307.052561 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 357 MS249-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (21, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.025086
2 0.021950
3 0.025086
4 0.128563
5 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.087799
1 0.119156
2 0.116020
3 0.015678
4 0.241448
...
354 0.006271
355 0.037628
356 0.003136
357 0.003136
358 0.015678
Name: Proten_Volume_Voxel_Resolved, Length: 359, dtype: float64
Cell Volume
0 117.729423
1 1603.719635
2 1477.367069
3 931.161499
4 1394.939227
5 109.582904
6 452.338737
7 472.131202
8 170.869935
9 70.521633
10 107.212324
11 82.844889
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 62057.742157
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
361 MS249-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.112885 2 0.003136 3 0.213227 4 0.065849 5 0.018814 6 0.040764 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.081528 1 0.047035 2 0.003136 3 0.200684 4 0.003136 5 0.003136 6 0.235177 7 0.009407 8 0.015678 9 0.137970 10 0.275941 11 0.021950 12 0.203820 13 0.457811 14 0.090935 15 0.012543 16 1.348346 17 0.194413 18 0.329247 19 0.056442 20 0.012543 21 0.037628 22 0.081528 23 0.003136 24 0.037628 25 0.021950 26 0.003136 27 1.326396 28 0.034493 29 0.087799 30 0.075257 31 0.297890 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1417.679240 1 1390.285865 2 1404.409006 3 1089.864962 4 474.100415 5 1371.249101 6 1024.946804 7 333.057148 8 80.389645 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 67038.193746 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 365 MS259-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (36, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.056442
2 0.015678
3 0.003136
4 0.003136
5 0.003136
6 0.015678
7 0.003136
8 0.003136
9 0.003136
10 0.006271
11 0.012543
12 0.003136
13 0.003136
14 0.009407
15 0.003136
16 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.012543
2 0.003136
3 0.003136
4 0.003136
...
1982 0.043900
1983 0.003136
1984 0.003136
1985 0.009407
1986 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 1987, dtype: float64
Cell Volume
0 572.996893
1 2178.698283
2 1322.993991
3 57.596325
4 1941.853463
5 1940.824957
6 1315.772501
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 109037.538959
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
368 MS259-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.181870
1 0.025086
2 0.006271
3 0.003136
4 0.003136
5 0.141106
6 0.228905
7 0.003136
8 0.028221
9 0.003136
10 0.059578
11 0.006271
12 0.006271
13 0.109749
14 0.043900
15 0.003136
16 0.003136
17 0.006271
18 0.003136
19 0.404504
20 0.015678
21 0.003136
22 0.018814
23 0.053307
24 0.018814
25 0.003136
26 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.128563
1 0.043900
2 0.003136
3 0.065849
4 0.006271
...
691 0.097206
692 0.034493
693 0.009407
694 0.028221
695 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 696, dtype: float64
Cell Volume
0 605.517118
1 206.792382
2 455.577903
3 1181.806483
4 1413.822343
5 1123.213009
6 1363.688956
7 1480.540386
8 1041.562817
9 564.292222
10 662.608597
11 1019.600455
12 1336.508808
13 1550.403524
14 820.612802
15 352.081370
16 1133.833586
17 358.305712
18 1648.688543
19 1179.542516
20 556.769705
21 1278.028219
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 80594.43967
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
371 MS274-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.003136 1 0.003136 2 0.272805 3 0.053307 4 0.774515 5 0.410775 6 0.003136 7 0.003136 8 0.003136 9 0.329247 10 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 674.289037 1 847.664387 2 1242.165350 3 966.811140 4 407.695941 5 1369.828634 6 487.116658 7 949.546040 8 1461.930075 9 495.322754 10 292.493883 11 954.842218 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 55610.44687 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 374 MS274-8_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [0 2 3 4 5 6] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.015678 1 0.003136 2 0.003136 3 0.003136 4 0.003136 5 0.003136 6 0.003136 7 0.006271 8 0.003136 9 0.006271 10 0.003136 11 0.043900 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1106.537418 1 1582.180591 2 661.514242 3 287.022106 4 909.274393 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 61213.624234 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 377 MS276-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (26, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.050171 2 0.015678 3 0.094071 4 0.062714 5 0.009407 6 0.009407 7 0.006271 8 0.125428 9 0.006271 10 0.006271 11 0.012543 12 0.047035 13 0.012543 14 0.009407 15 0.018814 16 0.047035 17 0.053307 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.034493 1 0.094071 2 0.047035 3 0.178734 4 0.015678 5 0.009407 6 0.087799 7 0.003136 8 0.081528 9 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1360.333769 1 563.614913 2 1829.711837 3 675.383392 4 1532.304331 5 1857.788792 6 546.415662 7 185.792676 8 1273.020524 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 75663.832984 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 380 MS276-8_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (41, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.012543 1 0.003136 2 0.006271 3 0.006271 4 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2362.556239 1 166.859389 2 681.635955 3 2582.277065 4 3175.975772 5 1327.816680 6 370.766938 7 3079.180206 8 100.668142 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 120960.577233 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 383 MS279-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 1 2 3 4 5 6 7 8 9 10 11 12 13] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Cell Volume 0 1031.948797 1 687.524778 2 1552.852497 3 868.372473 4 836.827447 5 1026.354728 6 826.485947 7 907.960540 8 341.319687 9 1176.949302 10 737.288153 11 992.752691 12 578.904530 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 54194.611415 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 386 MS279-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (16, 1024, 1024) Number of unique cells: [0 2 3 4 5] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Cell Volume 0 2926.773204 1 2112.503900 2 974.239586 3 775.330325 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 45819.275372 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 389 MS288-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (16, 1024, 1024) Number of unique cells: [0 2 3 4 5]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.006271 1 0.062714 2 0.551881 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.015678 1 0.301026 2 0.053307 3 0.006271 4 0.003136 5 0.018814 6 0.034493 7 0.072121 8 0.003136 9 0.003136 10 0.012543 11 0.006271 12 0.003136 13 0.003136 14 0.015678 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 913.266125 1 1078.454192 2 748.720873 3 968.225336 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 48899.455862 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 392 MS288-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 1.200969 1 0.025086 2 0.131699 3 0.021950 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1010.350175 1 731.512215 2 681.096616 3 1677.038302 4 1445.179227 5 1036.893777 6 1805.247196 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 47508.812528 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 395 MS290-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (17, 1024, 1024) Number of unique cells: [0 2 3 4 6 7] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1046.567376 1 172.444050 2 1273.001710 3 1285.864304 4 1733.515187 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 50384.737409 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 398 MS290-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8 9] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.003136
1 0.197548
2 2.047605
3 0.003136
4 0.003136
...
110 0.003136
111 0.003136
112 0.003136
113 0.003136
114 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 115, dtype: float64
Cell Volume
0 2074.314350
1 364.655481
2 829.314338
3 1507.899268
4 1019.920296
5 1829.890571
6 229.501037
7 2083.859386
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 55820.798258
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
401 MS292-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(15, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.028221
2 0.006271
3 0.003136
4 0.006271
5 0.225770
6 0.065849
7 0.003136
8 0.009407
9 0.065849
10 0.006271
11 0.031357
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.028221
1 0.053307
2 0.006271
3 0.003136
4 0.003136
...
364 0.003136
365 0.003136
366 0.009407
367 0.003136
368 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 369, dtype: float64
Cell Volume
0 717.850021
1 707.640219
2 602.701269
3 881.652114
4 859.953150
5 802.093426
6 1155.852390
7 823.224831
8 655.694404
9 1240.481485
10 247.013857
11 1215.057323
12 362.187694
13 300.844221
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 38747.868334
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
404 MS292-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(21, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.188141
1 0.003136
2 0.003136
3 0.003136
4 0.003136
...
159 0.003136
160 0.003136
161 0.112885
162 0.216363
163 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 164, dtype: float64
Cell Volume
0 1815.726667
1 1492.615922
2 1165.980664
3 577.230072
4 1305.995424
5 537.808197
6 1645.549719
7 779.180951
8 1713.904591
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 58014.168427
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
407 MS300-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(32, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.003136 1 0.006271 2 0.006271 3 0.003136 4 0.003136 5 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 65.680130 1 2056.431519 2 1812.757170 3 1748.362672 4 2344.554252 5 407.492121 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 96780.966912 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 410 MS300-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (36, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.018814 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.084664 1 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1023.034034 1 150.857971 2 266.734202 3 1163.603812 4 1048.025471 5 1532.796634 6 523.838705 7 1689.107567 8 2001.425272 9 51.789030 10 1036.758943 11 287.683737 12 202.239362 13 255.470809 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 107134.909823 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 413 MS301-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (41, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 9 10 11 12 13 14 15] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.172463 1 0.050171 2 0.003136 3 0.040764 4 0.040764 5 0.068985 6 0.097206 7 0.009407 8 0.047035 9 0.018814 10 0.003136 11 0.034493 12 0.003136 13 0.003136 14 0.021950 15 0.043900 16 0.147377 17 0.109749 18 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.021950 1 0.175599 2 0.003136 3 0.025086 4 0.003136 5 0.232041 6 0.003136 7 0.003136 8 0.072121 9 0.003136 10 0.037628 11 0.003136 12 0.018814 13 0.006271 14 0.003136 15 0.003136 16 0.172463 17 0.094071 18 0.003136 19 0.003136 20 0.003136 21 0.009407 22 0.012543 23 0.003136 24 0.006271 25 0.009407 26 0.003136 27 0.031357 28 0.025086 29 0.006271 30 0.003136 31 0.003136 32 0.003136 33 0.003136 34 0.018814 35 0.003136 36 0.006271 37 0.003136 38 0.003136 39 0.006271 40 0.006271 41 0.003136 42 0.018814 43 0.009407 44 0.003136 45 0.009407 46 0.003136 47 0.003136 48 0.003136 49 0.009407 50 0.003136 51 0.003136 52 0.003136 53 0.012543 54 0.003136 55 0.006271 56 0.006271 57 0.003136 58 0.081528 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 740.257650 1 1435.483679 2 2037.959178 3 2622.200650 4 1423.696626 5 391.750965 6 2501.834113 7 1893.353770 8 2455.253461 9 3896.578927 10 1480.753613 11 444.082469 12 58.919586 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 113426.188932 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 416 MS301-9_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (27, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 16 17 18 19 20 21 22] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
2 0.003136
3 0.181870
4 0.047035
5 0.043900
6 0.015678
7 0.006271
8 0.031357
9 0.003136
10 0.047035
11 0.072121
12 0.112885
13 0.094071
14 0.006271
15 0.006271
16 0.047035
17 0.003136
18 0.003136
19 0.021950
20 0.012543
21 0.006271
22 0.062714
23 0.003136
24 0.003136
25 0.003136
26 0.097206
27 0.172463
28 0.021950
29 0.009407
30 0.003136
31 0.009407
32 0.003136
33 0.003136
34 0.003136
35 0.087799
36 0.188141
37 0.009407
38 0.068985
39 0.169327
40 0.031357
41 0.006271
42 0.003136
43 0.012543
44 0.225770
45 0.006271
46 0.050171
47 0.006271
48 0.103478
49 0.068985
50 0.056442
51 0.006271
52 0.021950
53 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.028221
2 0.153649
3 0.021950
4 0.025086
...
330 0.034493
331 0.015678
332 0.003136
333 0.037628
334 0.047035
Name: Proten_Volume_Voxel_Resolved, Length: 335, dtype: float64
Cell Volume
0 844.704297
1 83.719746
2 824.065195
3 518.006324
4 193.832581
5 1028.377247
6 1301.759109
7 124.659294
8 293.566288
9 548.209276
10 162.184078
11 112.198068
12 946.068561
13 721.220886
14 1134.570472
15 855.058340
16 164.861956
17 75.212623
18 148.111108
19 108.196930
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 78587.624149
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
419 MS303-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(24, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19]
VoxelSize(x=0.10182914864199052, y=0.10182914864199052, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.018665 1 0.003111 2 0.006222 3 0.062215 4 0.031108 5 0.006222 6 0.003111 7 0.087101 8 0.208420 9 0.121319 10 0.003111 11 0.024886 12 0.015554 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.771467 1 0.012443 2 0.006222 3 0.003111 4 0.003111 5 0.136873 6 0.155538 7 0.003111 8 0.006222 9 0.003111 10 0.006222 11 0.087101 12 0.332851 13 0.009332 14 0.003111 15 0.006222 16 0.006222 17 0.003111 18 0.177313 19 0.003111 20 0.009332 21 0.003111 22 0.006222 23 0.006222 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1175.522320 1 1042.817612 2 1188.484827 3 283.513997 4 208.684842 5 769.180255 6 1684.189484 7 442.168604 8 245.976545 9 491.442926 10 1533.202882 11 798.184913 12 1072.911033 13 0.003111 14 374.391525 15 759.820000 16 805.989791 17 914.754147 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 64493.414984 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 422 MS303-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (27, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14] VoxelSize(x=0.10182914864199052, y=0.10182914864199052, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.018665 1 0.071547 2 0.003111 3 0.037329 4 0.059104 5 0.003111 6 0.003111 7 0.186645 8 0.015554 9 0.059104 10 0.111987 11 0.003111 12 0.003111 13 0.161759 14 0.121319 15 0.208420 16 0.009332 17 0.003111 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.006222 1 0.012443 2 0.006222 3 0.003111 4 0.003111 5 0.012443 6 0.006222 7 0.006222 8 0.009332 9 0.027997 10 0.009332 11 0.003111 12 0.012443 13 0.003111 14 0.021775 15 0.040440 16 0.003111 17 0.006222 18 0.009332 19 0.006222 20 0.015554 21 0.006222 22 0.015554 23 0.015554 24 0.012443 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 549.962405 1 1619.768907 2 1627.048068 3 1813.612348 4 679.295057 5 1284.479543 6 419.637422 7 818.311482 8 496.155716 9 237.932138 10 848.504448 11 637.383887 12 306.213159 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 76731.930942 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 426 MS304-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (41, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.003136
2 0.003136
3 0.018814
4 0.003136
5 0.003136
6 0.031357
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.263398
2 0.006271
3 0.003136
4 0.006271
...
512 0.003136
513 0.009407
514 0.003136
515 0.003136
516 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 517, dtype: float64
Cell Volume
0 1125.737238
1 154.730546
2 230.156396
3 1223.429612
4 1820.314179
5 2268.046590
6 1055.341032
7 1504.682051
8 2117.245061
9 220.824588
10 576.907096
11 1652.495269
12 378.349032
13 1270.151369
14 223.474244
15 342.514385
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 118643.91493
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
429 MS304-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(35, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 15 16 17 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.015678
2 0.006271
3 0.053307
4 0.003136
5 0.003136
6 0.028221
7 0.003136
8 0.009407
9 0.003136
10 0.003136
11 0.028221
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.028221
3 0.003136
4 0.037628
...
517 0.147377
518 0.009407
519 0.006271
520 0.009407
521 0.040764
Name: Proten_Volume_Voxel_Resolved, Length: 522, dtype: float64
Cell Volume
0 1995.514500
1 621.079539
2 1344.272773
3 1310.344624
4 986.512671
5 381.418871
6 1187.369194
7 1320.770788
8 975.747853
9 257.364765
10 1176.591833
11 184.660693
12 490.261753
13 1138.559068
14 507.511175
15 296.604770
16 68.389365
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 100837.293487
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
433 MS309-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.021950
1 0.031357
2 0.003136
3 0.078392
4 0.012543
5 0.021950
6 0.003136
7 0.028221
8 0.050171
9 0.009407
10 0.028221
11 0.003136
12 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.025086
1 0.003136
2 1.138255
3 0.003136
4 0.009407
...
2411 0.043900
2412 0.018814
2413 0.084664
2414 0.021950
2415 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 2416, dtype: float64
Cell Volume
0 1471.735373
1 659.689271
2 763.985405
3 878.946015
4 775.800679
5 571.610918
6 629.875145
7 201.367641
8 1292.352044
9 994.988437
10 202.041814
11 1443.661554
12 341.617578
13 1175.293658
14 816.492508
15 680.416172
16 697.944670
17 625.366025
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 67977.006325
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
436 MS309-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.068985
1 0.031357
2 0.028221
3 0.021950
4 0.100342
5 0.009407
6 0.034493
7 0.216363
8 0.006271
9 0.003136
10 0.018814
11 0.003136
12 0.003136
13 0.006271
14 0.003136
15 0.006271
16 0.003136
17 0.031357
18 0.003136
19 0.006271
20 0.021950
21 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 1.059863
1 0.003136
2 0.072121
3 3.007125
4 0.003136
...
2889 0.015678
2890 0.003136
2891 0.003136
2892 0.003136
2893 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 2894, dtype: float64
Cell Volume
0 1244.974927
1 810.315201
2 1029.352447
3 638.551595
4 670.695538
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 77806.301523
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
439 MS310-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(28, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.476625
1 0.018814
2 0.003136
3 0.003136
4 0.009407
...
341 0.006271
342 0.021950
343 0.003136
344 0.031357
345 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 346, dtype: float64
Cell Volume
0 1571.908076
1 1113.743230
2 2022.105138
3 1172.026271
4 337.506690
5 912.262704
6 724.776756
7 112.094591
8 64.171864
9 1501.339407
10 950.050886
11 729.759365
12 792.655004
13 355.841061
14 97.924415
15 2061.113102
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 77544.935619
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
442 MS310-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(33, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.006271
2 0.043900
3 0.021950
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.056442
1 0.053307
2 0.018814
3 0.006271
4 0.003136
...
138 0.003136
139 0.003136
140 0.003136
141 0.269669
142 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 143, dtype: float64
Cell Volume
0 897.339963
1 2256.758111
2 1308.184135
3 2126.589412
4 1078.965309
5 1980.030470
6 737.074926
7 943.751288
8 679.905055
9 2367.545120
10 465.179381
11 683.169306
12 865.835701
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 92113.924247
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
445 MS311-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(17, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells' 0 0.003136 1 0.006271 2 0.009407 3 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.062714 1 0.025086 2 0.062714 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1050.116975 1 322.756412 2 1168.448450 3 1590.844498 4 233.941172 5 1519.112489 6 259.158379 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 49751.75166 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 448 MS312-5_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (16, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.047035
1 0.003136
2 0.006271
3 0.006271
4 0.006271
5 0.012543
6 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.460946
2 0.084664
3 0.178734
4 0.003136
...
1092 0.003136
1093 0.003136
1094 0.021950
1095 0.003136
1096 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1097, dtype: float64
Cell Volume
0 1562.704830
1 1139.358669
2 831.869924
3 871.790373
4 759.940367
5 1212.009434
6 185.043247
7 886.750743
8 320.589651
9 299.107050
10 816.225974
11 49.098610
12 220.902980
13 179.728255
14 488.283134
15 313.597066
16 109.344592
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 42361.77749
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
451 MS312-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(17, 1024, 1024)
Number of unique cells:
[0 2 3 4]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 1.382839
1 0.279076
2 0.128563
3 0.034493
4 0.153649
5 0.009407
6 0.068985
7 0.056442
8 0.028221
9 0.043900
10 0.015678
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.012543
1 0.003136
2 0.003136
3 0.003136
4 0.015678
...
961 0.006271
962 0.003136
963 0.003136
964 0.003136
965 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 966, dtype: float64
Cell Volume
0 734.789010
1 534.562759
2 984.562273
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 53642.215995
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
454 MS313-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.062714
1 0.009407
2 0.028221
3 0.006271
4 0.012543
...
67 0.112885
68 0.003136
69 0.009407
70 0.003136
71 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 72, dtype: float64
Cell Volume
0 886.189455
1 184.892734
2 1082.066505
3 777.675820
4 1423.141609
5 1088.259490
6 429.213035
7 1346.778188
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 58541.936149
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
457 MS313-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.009407 1 0.003136 2 0.006271 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.015678 1 0.003136 2 0.031357 3 0.025086 4 0.031357 5 0.034493 6 0.075257 7 0.018814 8 0.009407 9 0.003136 10 0.009407 11 0.194413 12 0.043900 13 0.012543 14 0.040764 15 0.009407 16 0.097206 17 0.031357 18 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2486.390848 1 1905.962373 2 890.936887 3 1793.670234 4 260.393840 5 444.242389 6 555.007448 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 57423.548965 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 461 MS314-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (23, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.426454
1 0.545610
2 0.090935
3 0.006271
4 0.056442
...
3325 0.009407
3326 0.003136
3327 0.009407
3328 0.015678
3329 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 3330, dtype: float64
Cell Volume
0 1205.546780
1 619.213805
2 1451.212292
3 1016.894356
4 803.401008
5 843.638163
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 69684.269528
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
464 MS314-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(21, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.031357
1 0.012543
2 0.310433
3 0.003136
4 0.003136
5 0.009407
6 0.012543
7 0.021950
8 0.131699
9 0.147377
10 0.313569
11 0.009407
12 0.006271
13 0.003136
14 0.009407
15 0.003136
16 0.003136
17 0.028221
18 0.003136
19 0.012543
20 0.015678
21 0.015678
22 0.159920
23 0.006271
24 0.009407
25 0.040764
26 0.003136
27 0.006271
28 0.003136
29 0.003136
30 0.031357
31 0.062714
32 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.006271
3 0.021950
4 0.003136
...
2521 0.003136
2522 0.003136
2523 0.009407
2524 0.018814
2525 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 2526, dtype: float64
Cell Volume
0 554.483788
1 1458.687773
2 1198.419361
3 654.283344
4 1009.917450
5 1446.166969
6 191.556072
7 434.898038
8 438.908583
9 737.225439
10 444.436802
11 187.837145
12 600.089241
13 306.482189
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 59384.76844
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
467 MS315-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(24, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.448403
1 0.075257
2 0.119156
3 0.464082
4 0.021950
...
734 0.003136
735 0.003136
736 0.003136
737 0.006271
738 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 739, dtype: float64
Cell Volume
0 1327.365141
1 1079.187943
2 1131.252914
3 1234.981488
4 624.886265
5 65.002822
6 1024.830784
7 749.733701
8 1088.529159
9 262.836541
10 919.622165
11 308.360466
12 991.256968
13 1099.707888
14 199.210287
15 684.031621
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 66121.387429
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
471 MS315-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(27, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64)
Protein Volume Inside Void'
0 0.159920
1 0.003136
2 0.081528
3 0.150513
4 0.043900
...
713 0.003136
714 0.003136
715 0.012543
716 0.003136
717 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 718, dtype: float64
Cell Volume
0 1805.755177
1 1159.913106
2 1307.547590
3 1411.062937
4 1001.695674
5 682.570390
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 81407.661654
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
475 MS316-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
25]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.012543
2 0.003136
3 0.680444
4 0.021950
...
222 0.015678
223 0.003136
224 0.003136
225 0.028221
226 0.012543
Name: Proten_Volume_Voxel_Resolved, Length: 227, dtype: float64
Cell Volume
0 177.185211
1 138.870234
2 165.501636
3 1249.979486
4 192.208295
5 268.577987
6 2376.792265
7 1496.015008
8 247.829136
9 1424.477412
10 268.841385
11 1098.713875
12 550.100096
13 1578.781505
14 2766.087986
15 1651.946523
16 681.943252
17 486.150865
18 443.972720
19 1612.543462
20 375.909467
21 614.071276
22 1052.101866
23 2124.410109
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 78885.226068
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
478 MS316-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(41, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.025086
2 0.003136
3 0.018814
4 0.034493
5 0.031357
6 0.084664
7 0.003136
8 0.266534
9 0.003136
10 0.003136
11 0.040764
12 0.009407
13 0.329247
14 0.003136
15 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 1.169612
1 0.006271
2 0.006271
3 0.583238
4 0.006271
...
450 0.006271
451 0.006271
452 0.087799
453 0.028221
454 0.050171
Name: Proten_Volume_Voxel_Resolved, Length: 455, dtype: float64
Cell Volume
0 60.267932
1 1800.665955
2 1610.245003
3 1866.687875
4 1599.859603
5 330.887252
6 2014.808391
7 1880.654232
8 923.695424
9 125.988826
10 595.332402
11 307.325689
12 1913.895665
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 119777.99937
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
481 MS318-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.068985 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.928164 1 0.109749 2 0.037628 3 0.062714 4 0.040764 5 0.084664 6 0.059578 7 0.034493 8 0.062714 9 0.065849 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1510.865629 1 1090.012340 2 1173.324446 3 362.996702 4 136.747373 5 505.165680 6 1243.730059 7 786.029294 8 1115.345567 9 297.554884 10 660.391665 11 808.988805 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 56069.000541 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 484 MS318-7_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (25, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.034493 1 0.147377 2 0.047035 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.056442 1 0.442132 2 0.018814 3 0.040764 4 0.031357 5 0.021950 6 0.012543 7 0.025086 8 0.003136 9 0.003136 10 0.006271 11 0.009407 12 0.031357 13 0.003136 14 0.150513 15 0.018814 16 0.025086 17 0.087799 18 0.028221 19 0.003136 20 0.012543 21 0.012543 22 0.028221 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 488.229827 1 681.416456 2 337.939415 3 382.626111 4 1070.467593 5 608.953832 6 46.047585 7 48.697242 8 252.300628 9 273.190585 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 78010.321956 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 487 MS319-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.003136
1 0.003136
2 0.006271
3 0.006271
4 0.003136
5 0.003136
6 0.003136
7 0.225770
8 0.028221
9 0.031357
10 0.003136
11 0.003136
12 0.009407
13 0.006271
14 0.018814
15 0.156784
16 0.003136
17 0.012543
18 0.018814
19 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.006271
2 0.003136
3 0.003136
4 0.043900
...
166 0.021950
167 0.156784
168 0.043900
169 0.053307
170 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 171, dtype: float64
Cell Volume
0 2181.288362
1 907.741042
2 432.765770
3 801.275012
4 1431.774159
5 428.984129
6 744.039290
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 58832.285221
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
490 MS319-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.015678
1 0.050171
2 0.003136
3 0.018814
4 0.025086
5 0.025086
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.003136
2 0.285348
3 0.006271
4 0.006271
...
72 0.003136
73 0.018814
74 0.006271
75 0.075257
76 0.021950
Name: Proten_Volume_Voxel_Resolved, Length: 77, dtype: float64
Cell Volume
0 1479.173226
1 1302.110306
2 502.729250
3 1193.941597
4 1277.175312
5 253.514140
6 1065.538291
7 881.090825
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 57804.880037
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
493 MS320-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(16, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.003136 1 0.034493 2 0.112885 3 0.232041 4 0.056442 5 0.006271 6 0.009407 7 0.031357 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.003136 1 0.100342 2 0.009407 3 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 2148.031250 1 1384.582048 2 1060.364405 3 940.333387 4 335.920032 5 499.085580 6 309.069132 7 836.745919 8 538.993487 9 55.736862 10 140.115103 11 384.244127 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 43974.901055 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 496 MS320-4_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.015678 1 0.649088 2 0.065849 3 0.021950 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.799601 1 0.003136 2 0.031357 3 0.012543 4 0.003136 5 0.003136 6 0.021950 7 0.257126 8 0.003136 9 0.003136 10 0.003136 11 0.003136 12 0.003136 13 0.003136 14 0.003136 15 0.003136 16 0.003136 17 0.003136 18 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 72.857721 1 327.362738 2 742.211184 3 1186.334417 4 129.046122 5 1261.807303 6 1492.650415 7 464.034855 8 1398.946637 9 630.668475 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 58054.233118 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 499 MS321-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (18, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.147377
1 0.003136
2 0.034493
3 0.015678
4 0.003136
5 0.021950
6 0.009407
7 0.034493
8 0.169327
9 0.012543
10 0.009407
11 0.040764
12 0.037628
13 0.025086
14 0.006271
15 0.075257
16 0.021950
17 0.015678
18 0.015678
19 0.090935
20 0.050171
21 0.003136
22 0.012543
23 0.009407
24 0.003136
25 0.003136
26 0.003136
27 0.003136
28 0.003136
29 0.003136
30 0.257126
31 0.006271
32 0.003136
33 0.015678
34 0.003136
35 0.003136
36 0.009407
37 0.059578
38 0.006271
39 0.003136
40 0.012543
41 0.003136
42 0.021950
43 0.006271
44 0.003136
45 0.003136
46 0.003136
47 0.003136
48 0.053307
49 0.003136
50 0.028221
51 0.031357
52 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.018814
1 0.025086
2 0.050171
3 0.009407
4 0.072121
...
136 0.003136
137 0.003136
138 0.003136
139 0.003136
140 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 141, dtype: float64
Cell Volume
0 418.156597
1 103.794423
2 305.024094
3 316.641819
4 946.733327
5 451.746092
6 161.685503
7 629.241736
8 1190.191314
9 79.147912
10 1055.767486
11 198.275852
12 770.905869
13 171.155283
14 747.595161
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 51638.075217
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
502 MS321-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(17, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.351197 1 0.040764 2 0.282212 3 0.122292 4 0.003136 5 0.094071 6 0.012543 7 0.009407 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.501710 1 0.153649 2 0.078392 3 0.150513 4 0.009407 5 0.037628 6 0.025086 7 0.053307 8 0.112885 9 0.003136 10 0.313569 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 666.509394 1 259.255585 2 551.128601 3 785.035281 4 513.509747 5 273.058886 6 573.407668 7 564.935038 8 123.985121 9 558.795360 10 400.527757 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 50625.981599 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 506 MS324-2_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (18, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8 9] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.012543 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.015678 1 0.031357 2 0.003136 3 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1517.174634 1 599.543631 2 1485.673508 3 1119.594424 4 585.803044 5 1082.342446 6 913.642407 7 214.716266 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 51665.647325 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 509 MS324-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (20, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.410775 1 0.053307 2 0.028221 3 0.062714 4 0.097206 5 0.106613 6 0.426454 7 0.047035 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.442132 1 0.037628 2 1.332668 3 0.065849 4 0.050171 5 0.172463 6 0.037628 7 0.103478 8 0.053307 9 0.163056 10 0.175599 11 0.053307 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 433.138917 1 230.369623 2 513.766874 3 1525.901255 4 1195.681905 5 673.097475 6 1496.174928 7 1488.040953 8 406.852440 9 638.934149 10 1661.315961 11 1127.740943 12 287.461103 13 1573.780082 14 866.199441 15 977.365868 16 521.922799 17 837.012453 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 49305.395817 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 513 MS325-1_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (23, 1024, 1024) Number of unique cells: [0 2 3 4 5 6 7 8 9] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 2.119725
2 0.015678
3 4.540477
4 0.047035
5 0.253991
6 3.047889
7 0.003136
8 0.003136
9 0.003136
10 0.003136
11 0.006271
12 0.034493
13 0.003136
14 0.003136
15 0.003136
16 0.006271
17 0.003136
18 0.003136
19 0.003136
20 0.003136
21 0.003136
22 0.003136
23 0.062714
24 0.003136
25 0.100342
26 0.003136
27 0.003136
28 0.003136
29 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.188141
1 0.003136
2 0.009407
3 0.003136
4 0.003136
...
162 0.003136
163 0.003136
164 0.003136
165 0.012543
166 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 167, dtype: float64
Cell Volume
0 1434.524158
1 982.658910
2 799.277578
3 702.265649
4 1746.202182
5 1314.957222
6 1631.740147
7 74.024197
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 66938.525889
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
517 MS325-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.608324
1 0.137970
2 0.037628
3 0.009407
4 0.006271
5 0.025086
6 0.253991
7 0.489167
8 0.144242
9 0.009407
10 0.871721
11 0.025086
12 0.238312
13 0.025086
14 0.824686
15 0.003136
16 0.006271
17 0.012543
18 0.003136
19 0.097206
20 0.197548
21 0.021950
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.021950
2 0.003136
3 0.009407
4 0.050171
...
408 0.003136
409 0.006271
410 0.006271
411 0.003136
412 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 413, dtype: float64
Cell Volume
0 1024.818241
1 1020.500398
2 1526.907811
3 1280.248286
4 1985.652759
5 444.016620
6 582.588964
7 804.950039
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 73530.508113
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
521 MS62-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(15, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.006271
1 0.003136
2 0.125428
3 0.003136
4 0.078392
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.015678
1 0.486032
2 0.037628
3 0.144242
4 0.059578
...
87 0.003136
88 0.031357
89 0.003136
90 0.003136
91 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 92, dtype: float64
Cell Volume
0 609.025953
1 872.285812
2 683.344905
3 477.317631
4 412.374388
5 337.669746
6 543.145139
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 45384.951165
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
524 MS62-6_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(14, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' Series([], Name: Proten_Volume_Voxel_Resolved, dtype: float64) Protein Volume Inside Void' 0 0.003136 1 0.040764 2 0.003136 3 0.006271 4 0.003136 5 0.003136 6 0.006271 7 0.003136 8 0.097206 9 0.059578 10 0.003136 11 0.003136 12 0.003136 13 0.003136 14 0.003136 15 0.003136 16 0.003136 17 0.003136 18 0.003136 19 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 715.611139 1 561.852656 2 352.065692 3 374.313401 4 520.220120 5 341.435708 6 351.363298 7 189.198034 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 42626.047041 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 527 MS65-3_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (32, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.006271
2 0.109749
3 0.006271
4 0.003136
5 0.003136
6 0.003136
7 0.003136
8 0.003136
9 0.003136
10 0.028221
11 0.003136
12 0.012543
13 0.003136
14 0.003136
15 0.003136
16 0.003136
17 0.003136
18 0.003136
19 0.006271
20 0.012543
21 0.003136
22 0.003136
23 0.003136
24 0.003136
25 0.003136
26 0.003136
27 0.003136
28 0.003136
29 0.021950
30 0.003136
31 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.730615
1 0.144242
2 1.219783
3 0.072121
4 0.122292
...
752 0.003136
753 0.003136
754 0.003136
755 0.006271
756 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 757, dtype: float64
Cell Volume
0 220.115922
1 652.599480
2 1891.582106
3 36.217202
4 1608.206805
5 612.349783
6 40.647929
7 1547.189444
8 1573.206251
9 1420.633058
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 95613.496796
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
531 MS65-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(33, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.021950
1 0.072121
2 0.087799
3 0.003136
4 0.003136
5 0.040764
6 0.056442
7 0.003136
8 0.006271
9 0.006271
10 0.003136
11 0.003136
12 0.015678
13 0.015678
14 0.322976
15 0.009407
16 0.003136
17 0.137970
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.141106
1 0.131699
2 0.156784
3 0.025086
4 0.473489
...
544 0.006271
545 0.009407
546 0.006271
547 0.003136
548 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 549, dtype: float64
Cell Volume
0 4218.385226
1 186.062345
2 588.364902
3 2290.905758
4 3859.458648
5 2360.881782
6 2947.490698
7 2131.973389
8 573.812172
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 89346.917505
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
535 MS69-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(35, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.216363
1 0.056442
2 0.006271
3 0.003136
4 0.097206
5 0.003136
6 0.213227
7 0.003136
8 0.003136
9 0.003136
10 0.003136
11 0.003136
12 0.003136
13 0.003136
14 0.003136
15 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 1.163340
1 0.840365
2 0.015678
3 0.370011
4 0.385690
...
952 0.003136
953 0.009407
954 0.003136
955 0.003136
956 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 957, dtype: float64
Cell Volume
0 1443.376206
1 3130.853216
2 1436.487099
3 172.672956
4 1458.211148
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 107438.667098
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
539 MS69-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.006271
2 0.003136
3 0.087799
4 0.012543
5 0.006271
6 0.003136
7 0.031357
8 0.009407
9 0.141106
10 0.087799
11 0.109749
12 0.003136
13 0.003136
14 0.003136
15 0.006271
16 0.012543
17 0.241448
18 0.006271
19 0.003136
20 0.100342
21 0.006271
22 0.003136
23 0.028221
24 0.006271
25 0.429589
26 0.003136
27 0.003136
28 0.003136
29 0.166191
30 0.006271
31 0.003136
32 0.003136
33 0.003136
34 0.006271
35 0.003136
36 0.003136
37 0.128563
38 0.009407
39 0.003136
40 0.009407
41 0.009407
42 0.003136
43 0.003136
44 0.003136
45 0.018814
46 0.003136
47 0.633409
48 0.003136
49 0.006271
50 0.003136
51 0.379418
52 0.003136
53 0.012543
54 0.009407
55 0.012543
56 0.043900
57 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.225770
1 0.097206
2 0.185006
3 0.109749
4 0.006271
...
1479 0.015678
1480 0.003136
1481 0.003136
1482 0.003136
1483 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1484, dtype: float64
Cell Volume
0 375.683697
1 303.562863
2 1757.258620
3 2195.571422
4 382.983580
5 1955.198953
6 1988.340044
7 1508.121901
8 1817.971820
9 1530.394696
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 84825.141879
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
543 MS70-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(12, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.068985
1 0.031357
2 0.100342
3 0.194413
4 0.003136
5 0.012543
6 0.028221
7 0.003136
8 0.006271
9 0.003136
10 0.028221
11 0.003136
12 0.018814
13 0.003136
14 0.040764
15 0.040764
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.025086
2 0.037628
3 0.034493
4 0.059578
...
367 0.040764
368 0.003136
369 0.003136
370 0.009407
371 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 372, dtype: float64
Cell Volume
0 526.353527
1 689.051858
2 852.656403
3 693.143931
4 491.675949
5 175.099979
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 36028.110145
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
546 MS70-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(16, 1024, 1024)
Number of unique cells:
[0 2 3 4 5]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.147377
1 0.244584
2 0.003136
3 0.391961
4 0.003136
5 0.068985
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.304162
1 0.003136
2 0.301026
3 0.203820
4 0.034493
...
375 0.003136
376 0.003136
377 0.003136
378 0.006271
379 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 380, dtype: float64
Cell Volume
0 418.614408
1 603.629433
2 1268.865737
3 1151.873201
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 49165.139608
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
549 MS71-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7 8 9]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.018814
2 0.009407
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.009407
1 0.040764
2 0.050171
3 0.119156
4 0.787058
...
87 0.006271
88 0.015678
89 0.021950
90 0.090935
91 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 92, dtype: float64
Cell Volume
0 847.633030
1 360.575950
2 991.746135
3 1458.756758
4 236.559472
5 1176.651411
6 740.442656
7 494.068479
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 59453.719093
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
552 MS71-4_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(26, 1024, 1024)
Number of unique cells:
[0 2 3 4 5 6 7]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.047035
2 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.003136
3 0.003136
4 0.141106
...
283 0.047035
284 0.037628
285 0.003136
286 0.037628
287 0.009407
Name: Proten_Volume_Voxel_Resolved, Length: 288, dtype: float64
Cell Volume
0 901.987053
1 591.058458
2 1859.779954
3 89.053552
4 1463.469698
5 266.828273
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 80316.021892
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
555 MS74-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(25, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.003136
2 0.003136
3 0.003136
4 0.003136
...
56 0.006271
57 0.012543
58 0.034493
59 0.009407
60 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 61, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 1.984891
2 0.003136
3 0.094071
4 0.003136
...
3137 0.003136
3138 0.006271
3139 0.003136
3140 0.003136
3141 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 3142, dtype: float64
Cell Volume
0 1779.029705
1 343.959937
2 661.351186
3 1112.689638
4 1920.179585
5 1042.268347
6 588.026247
7 13.022514
8 324.449684
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 74415.214387
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
558 MS74-5_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[0 2 3 4 5]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.031357
1 0.181870
2 1.200969
3 0.025086
4 1.141391
...
56 0.043900
57 0.003136
58 0.156784
59 0.003136
60 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 61, dtype: float64
Protein Volume Inside Void'
0 0.467218
1 0.395097
2 0.740022
3 0.279076
4 0.025086
...
4395 0.003136
4396 0.003136
4397 0.003136
4398 0.003136
4399 0.006271
Name: Proten_Volume_Voxel_Resolved, Length: 4400, dtype: float64
Cell Volume
0 3329.345430
1 669.961787
2 1330.356588
3 32.598617
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 93277.967056
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
561 MS80-2_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(29, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.003136
1 0.009407
2 0.031357
3 0.003136
4 0.025086
5 0.065849
6 0.028221
7 0.037628
8 0.006271
9 0.018814
10 0.025086
11 0.009407
12 0.050171
13 0.109749
14 0.172463
15 0.072121
16 0.025086
17 0.009407
18 0.250855
19 0.094071
20 0.003136
21 0.006271
22 0.043900
23 0.003136
24 0.003136
25 0.043900
26 0.018814
27 0.003136
28 0.003136
29 0.112885
30 0.291619
31 0.006271
32 1.373432
33 0.012543
34 0.037628
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 4.054445
1 1.131984
2 0.849772
3 0.034493
4 0.028221
...
790 0.003136
791 0.003136
792 0.009407
793 0.003136
794 0.018814
Name: Proten_Volume_Voxel_Resolved, Length: 795, dtype: float64
Cell Volume
0 973.756690
1 130.598288
2 619.185584
3 818.182644
4 1533.674627
5 492.149437
6 77.300992
7 738.100297
8 83.986279
9 875.888718
10 1420.774164
11 1085.114394
12 134.684090
13 123.132214
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 86245.69341
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
564 MS80-9_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(21, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.021950
1 0.194413
2 0.529931
3 0.087799
4 0.266534
5 1.037913
6 0.025086
7 0.025086
8 0.081528
9 0.018814
10 0.037628
11 0.238312
12 0.081528
13 0.028221
14 0.021950
15 0.021950
16 0.059578
17 0.003136
18 0.125428
19 0.294755
20 0.003136
21 0.006271
22 0.068985
23 0.018814
24 0.018814
25 0.053307
26 0.003136
27 0.068985
28 0.003136
29 0.119156
30 0.065849
31 0.172463
32 0.021950
33 0.006271
34 0.009407
35 0.006271
36 0.087799
37 0.301026
38 0.006271
39 0.003136
40 0.003136
41 0.040764
42 0.012543
43 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 6.374855
1 0.047035
2 0.658495
3 4.709804
4 0.285348
...
654 0.003136
655 0.003136
656 0.031357
657 0.021950
658 0.025086
Name: Proten_Volume_Voxel_Resolved, Length: 659, dtype: float64
Cell Volume
0 1439.942628
1 2024.905307
2 601.879719
3 695.185264
4 286.087671
5 957.639252
6 1204.342676
7 220.601954
8 109.175265
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 61508.400898
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
567 MS81-3_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
Protein Volume Inside Cells'
0 0.087799
1 0.003136
2 0.012543
3 0.003136
4 0.072121
5 0.003136
6 0.003136
7 0.006271
8 0.009407
9 0.003136
10 0.012543
11 0.040764
12 0.006271
13 0.003136
14 0.015678
15 0.021950
16 0.009407
17 0.006271
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.040764
1 0.015678
2 0.006271
3 0.006271
4 0.006271
...
206 0.003136
207 0.009407
208 0.003136
209 0.015678
210 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 211, dtype: float64
Cell Volume
0 1118.901437
1 430.150606
2 1512.709414
3 440.407442
4 1075.130362
5 1498.040663
6 848.432630
7 587.571573
8 915.730776
9 553.671645
10 933.369023
11 95.202637
12 170.311782
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 55580.522995
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
570 MS81-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 9 10 11 12 13 14 15 16]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells' 0 0.200684 1 0.012543 2 0.163056 3 0.006271 4 0.003136 5 0.216363 6 0.006271 7 0.047035 8 0.009407 9 0.028221 10 0.059578 11 0.144242 12 0.078392 13 0.006271 14 0.059578 15 0.043900 16 0.003136 17 0.056442 18 0.018814 19 0.003136 20 0.003136 21 0.006271 22 0.018814 23 0.003136 24 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Protein Volume Inside Void' 0 0.031357 1 0.034493 2 0.009407 3 0.163056 4 0.012543 5 0.012543 6 0.003136 7 0.009407 8 0.144242 9 0.018814 10 0.003136 11 0.003136 12 0.012543 13 0.003136 14 0.106613 15 0.003136 16 0.003136 17 0.003136 18 0.009407 19 0.003136 20 0.094071 21 0.003136 22 0.015678 23 0.003136 24 0.003136 25 0.144242 26 0.015678 27 0.003136 28 0.021950 29 0.003136 30 0.009407 31 0.012543 32 0.003136 33 0.003136 34 0.006271 35 0.012543 36 0.009407 37 0.003136 38 0.003136 39 0.003136 40 0.003136 41 0.003136 42 0.003136 43 0.003136 44 0.003136 45 0.031357 46 0.003136 Name: Proten_Volume_Voxel_Resolved, dtype: float64 Cell Volume 0 1477.103671 1 1033.776903 2 669.594911 3 2141.198585 4 1177.043372 5 1205.941877 6 1319.290743 7 405.638929 8 315.268388 9 1526.136432 10 802.902434 11 1041.465611 12 1037.853298 13 186.513885 Name: Cell_Volume_Voxel_Resolved, dtype: float64 Void Volume 0 61284.446894 Name: Void_Volume_Voxel_Resolved, dtype: float64 Move On to Next Image Images from folder 573 MS92-6_Python Name: IMAGE_ID, dtype: object shape of image Z,X, Y: (30, 1024, 1024) Number of unique cells: [ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16] VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.043900
1 0.263398
2 0.012543
3 0.250855
4 0.037628
5 0.119156
6 0.034493
7 0.147377
8 0.003136
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.391961
1 0.015678
2 0.520524
3 0.068985
4 0.094071
...
110 0.050171
111 0.012543
112 0.003136
113 0.087799
114 0.021950
Name: Proten_Volume_Voxel_Resolved, Length: 115, dtype: float64
Cell Volume
0 1471.882750
1 1636.782334
2 2184.593377
3 615.422757
4 1245.658507
5 2035.478849
6 2368.918551
7 2108.151564
8 38.198957
9 209.081434
10 770.755356
11 156.646452
12 451.604986
13 653.784770
14 603.861474
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 82089.407358
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
576 MS92-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(31, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.090935
1 0.012543
2 0.454675
3 0.003136
4 0.003136
5 0.003136
6 0.119156
7 0.006271
8 0.047035
9 0.294755
10 0.050171
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.185006
1 0.526796
2 0.018814
3 0.043900
4 0.003136
...
161 0.003136
162 0.018814
163 0.003136
164 0.043900
165 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 166, dtype: float64
Cell Volume
0 772.799825
1 1905.667618
2 2164.929475
3 354.812555
4 2004.642489
5 2209.904654
6 341.799448
7 198.426365
8 1373.550696
9 745.030168
10 420.445650
11 2922.634096
12 879.096528
13 400.621827
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 85233.875733
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
579 MS94-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(20, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.119156
1 0.047035
2 0.012543
3 0.012543
4 0.068985
5 0.021950
6 0.003136
7 0.003136
8 0.094071
9 0.078392
10 0.147377
11 0.006271
12 0.003136
13 0.116020
14 0.015678
15 0.043900
16 0.075257
17 0.003136
18 0.012543
19 0.018814
20 0.006271
21 0.003136
22 0.003136
23 0.065849
24 0.156784
25 0.068985
26 0.034493
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.003136
1 0.003136
2 0.009407
3 0.003136
4 0.003136
...
357 0.003136
358 0.003136
359 0.009407
360 0.009407
361 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 362, dtype: float64
Cell Volume
0 974.277215
1 157.483681
2 808.509045
3 387.746691
4 578.324427
5 933.375295
6 94.838897
7 849.545800
8 473.570483
9 1223.548768
10 192.518728
11 603.453835
12 606.978348
13 93.863698
14 108.438378
15 65.576652
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 57608.103044
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
582 MS94-7_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(23, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.012543
1 0.034493
2 0.003136
3 0.047035
4 0.025086
5 0.025086
6 0.103478
7 0.003136
8 0.357468
9 0.181870
10 0.159920
11 0.018814
Name: Proten_Volume_Voxel_Resolved, dtype: float64
Protein Volume Inside Void'
0 0.112885
1 0.040764
2 0.094071
3 0.163056
4 0.006271
...
73 0.003136
74 0.009407
75 0.028221
76 0.116020
77 0.025086
Name: Proten_Volume_Voxel_Resolved, Length: 78, dtype: float64
Cell Volume
0 1372.359134
1 510.044811
2 1199.783385
3 680.366001
4 768.892757
5 155.279292
6 126.788427
7 353.975326
8 1238.976355
9 1319.541598
10 169.088864
11 605.466947
12 1158.081864
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 65965.531171
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
585 MS99-1_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(30, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 9 10 11 12 13 14 16 17 18]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.818415
1 0.025086
2 0.003136
3 0.090935
4 0.050171
...
113 0.006271
114 0.006271
115 0.003136
116 0.003136
117 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 118, dtype: float64
Protein Volume Inside Void'
0 0.006271
1 0.072121
2 0.006271
3 0.003136
4 0.006271
...
1868 0.003136
1869 0.003136
1870 0.003136
1871 0.028221
1872 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 1873, dtype: float64
Cell Volume
0 1470.581439
1 2165.635005
2 232.853088
3 2479.250885
4 885.060607
5 85.695230
6 1259.565285
7 566.114057
8 601.788784
9 88.122252
10 365.781193
11 542.342402
12 164.328889
13 251.425771
14 635.045896
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 86846.638693
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
Images from folder
588 MS99-8_Python
Name: IMAGE_ID, dtype: object
shape of image Z,X, Y:
(37, 1024, 1024)
Number of unique cells:
[ 0 2 3 4 5 6 7 8 9 10 11 12 13 14 15]
VoxelSize(x=0.1022364652365585, y=0.1022364652365585, z=0.3)
C:\Users\richa\AppData\Local\Temp\ipykernel_22212\412934641.py:27: UserWarning: ND2File file not closed before garbage collection. Please use `with ND2File(...):` context or call `.close()`. nd2_img=nd2.ND2File(nd2_path[0])
Protein Volume Inside Cells'
0 0.006271
1 0.043900
2 0.015678
3 0.012543
4 0.009407
...
63 0.003136
64 0.006271
65 0.018814
66 0.003136
67 0.084664
Name: Proten_Volume_Voxel_Resolved, Length: 68, dtype: float64
Protein Volume Inside Void'
0 109.125094
1 0.288483
2 1.379703
3 146.016468
4 2.844069
...
3586 0.003136
3587 0.003136
3588 0.003136
3589 0.003136
3590 0.003136
Name: Proten_Volume_Voxel_Resolved, Length: 3591, dtype: float64
Cell Volume
0 2408.895443
1 1198.707844
2 736.218883
3 1503.158107
4 2327.041432
5 1760.500922
6 855.854805
7 2232.748145
8 38.236585
9 235.505881
10 1432.341719
11 834.933491
12 773.828331
13 338.967921
Name: Cell_Volume_Voxel_Resolved, dtype: float64
Void Volume
0 104979.343513
Name: Void_Volume_Voxel_Resolved, dtype: float64
Move On to Next Image
No more images left
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